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使用Diazomethane (TrEnDi) 的三甲基化增强使得从复杂的甘油样本中能够更好地检测出Glufosinate和3- ((Methylphosphinico) propionic酸

Christian A Rosales^1,2, Krysten L Sheedy^1,2, Karl V Wasslen^1,2

¹Department of Chemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada.

Journal of the American Society for Mass Spectrometry

|December 21, 2023

概括

使用Diazomethane (TrEnDi) 的三甲基化增强改善了除草剂葡萄酸盐 (GLUF) 和其代谢物MPPA的检测. 这种新的方法可以在植物样本中量化,而未衍生形式无法检测到.

科学领域:

农业化学农业化学
分析化学分析化学
环境科学环境科学

背景情况:

现代农业在很大程度上依赖于除草剂,如葡萄酸盐 (GLUF) 来控制杂草.
越来越多的GLUF使用引发了人们对其在食物链中的持久性和检测的担忧.
分解产物3-基酸 (MPPA) 也需要敏感的检测方法.

研究的目的:

引入和评估使用Diazomethane (TrEnDi) 的三甲基化增强剂,以改善GLUF和MPPA的检测.
评估TrEnDi在色谱保留和质谱学灵敏度方面的分析优势.
为了证明TrenDi在农业样本中量化GLUF和MPPA的适用性.

主要方法:

使用Diazomethane (TrEnDi) 来形成甲基分析物的GLUF和MPPA的定量衍生.
染色体保留和MS敏感性对衍生与非衍生分析物的比较.
在田间种植的甘露植物的水性提取物中应用TrEnDi,以量化分析物.

主要成果:

TrEnDi显著改善了逆相色谱保留 (GLUF的2.8倍,MPPA的1.7倍).
基于MS的敏感性显示显著增强 (GLUF的4.1倍,MPPA的11.0倍).
在未衍生化的现场样本中无法检测到GLUF和MPPA,但在TrEnDi衍生后可以量化.

结论:

在检测GLUF和MPPA方面,TrEnDi提供了显著的分析优势.
该方法可以在复杂的农业矩阵中对这些化合物进行敏感的量化.
在环境和食品安全分析中,TrEnDi是提高具有挑战性的有机分子检测能力的宝贵工具.

关键词:

3-(甲基) 酸是一种酸.这是一个MRMM.这是一个趋势.亚甲 (diazomethane) 是一种葡萄酸的使用方法葡萄酸-氨基的使用这是一种除草剂除草剂.质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量双联质谱质量测量技术

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