在雌性中,TAC3 调节了 GnRH/淋巴激素合成
Fengyan Meng1, Jinxuan Li1, Xingfa Han1
1College of Life Science, Sichuan Agricultural University, Xinkang Road, Ya'an, 625014, PR China.
Theriogenology
|December 21, 2023
概括
神经基因因B (NKB) 通过激活在下丘脑和垂体中的塔基基因因受体3 (TACR3) 调节雌性的繁殖,影响淋巴激素合成.
科学领域:
- * 生殖内分泌学 生殖内分泌学
- * 神经内分泌学 * 神经内分泌学
- * 分子生物学 * 分子生物学
背景情况:
- *神经蛋白B (NKB) 对于许多物种的繁殖至关重要,但它在鸟类中的作用尚不清楚.
- * 塔基宁3 (TAC3) 基因编码NKB,塔基宁受体3 (TACR3) 是其主要受体.
研究的目的:
- * 为了研究NKB-TACR3系统在雌性的生殖轴中的功能.
- * 为了确定NKB是否影响的淋巴激素合成和释放.
主要方法:
- * TAC3 (cTAC3) 和其编码的NKB的克隆和特征.
- *基于细胞的测定以确认cNKB激活cTACR3.
- *对在下丘脑和垂体中cTAC3和cTACR3表达的定量分析.
- * 在体内 (内注射) 和体外 (培养下垂体细胞) 实验中评估cNKB对基因表达的影响.
主要成果:
- * TAC3cDNA被克隆,预测一个10氨基酸成熟的NKB.
- * cNKB在HEK293细胞中特别激活了cTACR3.
- *cTAC3和cTACR3在雌性的下丘脑和垂体中表达高.
- * cNKB的使用增加了下丘脑GnRH-I和垂体FSHβ和LHβ的表达.
- * cNKB在培养下垂体细胞中直接刺激LHβ和FSHβ的表达.
结论:
- *NKB-TACR3信号通路在雌性的生殖神经内分泌系统中存在和功能.
- *NKB在下丘脑和垂体水平上都起作用,刺激性腺激素的合成,从而调节生殖.
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