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PLZF蛋白与蛋白TET1形成复合体,以向未分化精子的TCF7L2

Xiaomin Du¹, Donghui Yang², Xiuwei Yu³

¹College of Veterinary Medicine, Northwest A&F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, 712100, China; Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, College of Life Sciences, Yulin University, Yulin, Shaanxi, 719000, China.

|December 21, 2023

概括

亲细胞白血病指 (PLZF) 通过通过TET1和H3K4me3.3.2通过表观遗传控制TCF7L2表达来调节男性生殖细胞的自我更新. 这揭示了一个新的调节轴,对于精子干细胞维护至关重要.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
干细胞生物学干细胞生物学

背景情况:

孕细胞白血病的指 (PLZF,ZBTB16) 对于精子干细胞 (SSC) 的自我更新至关重要.
在SSC中PLZF的确切功能仍然不完全理解.

研究的目的:

阐明PLZF作为胚胎细胞维护和自我更新中的表观遗传调节者的作用.
为了确定基于PLZF介导的SSC功能调节的分子机制.

主要方法:

蛋白质与蛋白质相互作用试验 (PLZF和TET1).
对与PLZF和TCF7L2.2.相关的基因表达和基因素甲基化 (H3K4me3) 的分析.
丸单细胞测序以确定TCF7L2表达模式.
基因组分析以确定PLZF目标和相互作用 (例如,与EZH2).

主要成果:

PLZF与TET1相互作用,调节自我更新基因表达.
在与H3K4me3的协调下,PLZF促进了转录因子7-like 2 (TCF7L2) 的表达.
PLZF直接准TCF7L2,改变了SSC中的基因组甲基化格局.
建立了一个新型的PLZF-TET1-H3K4me3-TCF7L2轴,调节不分化的精子.
确定了PLZF和EZH2之间的一种新的基因组关联.

结论:

在SSC的维护和自我更新中,PLZF作为一个关键的表观遗传调节器.
已确定的PLZF-TET1-H3K4me3-TCF7L2轴对于调节不分化的精子来说至关重要.
这项研究为进一步研究男性生殖细胞发育提供了基础,特别是在家禽中.

关键词:

希斯甲基化希斯甲基化在PLZF中.精子干细胞 (Spermatogonial stem cells) 是一种精子干细胞.在TCF7L2中使用.测试 (Testis) 是一个测试.

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