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一个基于菌体编码蛋白RBP 41的电化学生物传感器,用于快速和灵敏地检测沙门氏菌

Yifeng Ding¹, Yiming Zhang¹, Chenxi Huang¹

¹Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

|December 21, 2023

概括

使用RBP 41的新电化学生物传感器可以快速准确地检测食品中的沙门氏菌. 这一进步提供了一个有希望的工具,通过确保食品安全来预防食源性疾病.

科学领域:

食品安全食品安全
生物传感器技术的技术
微生物检测检测微生物检测

背景情况:

沙门氏菌引起的沙门氏菌病是与受污染食品相关的重大公共卫生问题.
快速准确地检测沙门氏菌对于预防食品传播疫情和确保消费者安全至关重要.

研究的目的:

开发一种新的电化学生物传感器,用于敏感和特定检测沙门氏菌.
为了利用一个新发现的生物识别元素,RBP 41,捕获沙门氏菌.

主要方法:

使用石墨烯氧化物 (GO),金纳米粒子 (GNP) 和RBP 41在玻璃碳电极 (GCE) 上层层组装的生物传感器的制造.
使用微分脉冲电压计 (DPV) 进行信号检测和量化.
测试生物传感器的性能,在不同的沙门氏菌度和真实食品矩阵 (脱脂牛奶,菜).

主要成果:

生物传感器在大约30分钟内检测到沙门氏菌,其度范围为3至106CFU/mL.
取得了0.2984 Log10 CFU/mL的令人印象深刻的检测极限.
生物传感器在检测脱脂牛奶和生菜样本中的沙门氏菌方面表现出卓越的特异性和有效性.

结论:

开发的基于RBP 41的电化学生物传感器提供了快速,灵敏和特定的沙门氏菌检测方法.
尾受体结合蛋白显示出生物感知应用的巨大潜力.
这种生物传感器代表了在各种食品样本中进行常规沙门氏菌监测的有希望的替代方案,增强了食品安全协议.

关键词:

微分脉冲电压测量微分脉冲电压测量电化学生物传感器菌体受体结合蛋白是一种菌体受体结合蛋白.快速和敏感的检测检测.沙门氏菌是一种沙门氏菌.

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