加密球菌蛋白酶具有激活潜在的SARS-CoV-2尖端蛋白的潜能
Nozethu Mjokane1, Saheed Sabiu2, Olufemi S Folorunso3
1Department of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein, 9301, South Africa.
Journal of infection and public health
|December 21, 2023
概括
加密球菌蛋白酶可以激活SARS-CoV-2尖端蛋白,可能会使COVID-19恶化. 这一发现强调了在COVID-19患者中查加密球菌感染的重要性.
科学领域:
- *病毒学和传染病研究.
- *分子生物学和蛋白质相互作用.
背景情况:
- *COVID-19疫情在全球造成了数百万人的死亡.
- *COVID-19可能与微生物感染同时发生,包括像加密球菌病这样的肺部感染.
- * 密码球菌病涉及的真菌病原体,可以导致原发性肺部感染.
研究的目的:
- * 为了研究含有分泌蛋白酶的密码球菌超级生物是否可以激活SARS-CoV-2尖端蛋白.
- * 为了比较加密球菌蛋白酶的酶活性与激活尖端蛋白的人类蛋白酶.
主要方法:
- *使用HADDOCK服务器将SARS-CoV-2尖端蛋白与密码球菌蛋白酶进行基分子对接.
- * 生物化学测试评估了加密球菌蛋白酶在通过SARS-CoV-2尖端伪型Lentivirus.通过HEK-293T细胞中介病毒进入病毒的效率.
- *使用人类蛋白酶作为参考配体进行比较分析.
主要成果:
- * 选择的密码球菌蛋白酶与SARS-CoV-2尖端蛋白相互作用.
- * 一些加密球菌蛋白酶表现出与人类furin相比或更高的结合亲和力.
- * 密码球菌超体在病毒入口转导中显示出类似于重组的生化效率.
结论:
- * 加密球菌蛋白酶具有激活SARS-CoV-2尖端蛋白的能力.
- * 存在密码球菌感染可能通过分泌的蛋白酶增强SARS-CoV-2病毒粒子激活.
- * 对密码球菌感染的查对于管理COVID-19患者和优化干预策略至关重要.
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