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通过多电极圆柱形DBD等离子体生成的氧化水减轻水中传播的致病细菌中T3SS介导的毒性

Shweta B Borkar¹, Manorma Negi¹, Tirtha Raj Acharya¹

¹Department of Electrical and Biological Physics /Plasma Bioscience Research Center, Kwangwoon University, Seoul, 01897, South Korea.

|December 21, 2023

概括

多电极圆柱形DBD血产生的氧化水 (MCDBD-PG-NOW) 有效抑制了腹病原体的生长和毒性. 这种创新的治疗方法破坏了细菌感染机制,并减少了对宿主细胞的粘附.

科学领域:

微生物学微生物学
血医学是一种血医学.
食品安全食品安全

背景情况:

沙门氏菌 (Salmonella enterica),柔氏菌 (Shigella flexneri) 和血过杆菌 (Vibrio parahaemolyticus) 是全球范围内导致腹疾病的主要原因.
* 这些细菌利用III型分泌系统 (T3SS) 来侵入宿主细胞并产生毒性.
* 开发新的抗微生物策略对于公共卫生至关重要.

研究的目的:

*研究多电极圆柱式DBD血产生的氧化水 (MCDBD-PG-NOW) 对病原性细菌的疗效.
*阐明MCDBD-PG-NOW影响细菌生存,毒性和宿主细胞相互作用的机制.

主要方法:

*采用了殖民地形成单元 (CFU) 试验,活细胞/死细胞染色,脂质过氧化试验和形态分析.
*生物素切换试验,DAF-FM和FTIR分析被用于研究反应性物种 (RNS) 相互作用.
* 量化PCR用一氧化评估了可活但不能培养 (VBNC) 细菌的诱导.

主要成果:

*MCDBD-PG-NOW显著抑制了细菌的生长,并减少了毒性.
*RNA与细菌膜相互作用,形成S-尼特罗斯醇,并在细胞内积累.
* 细菌对结肠细胞的粘附和毒性基因表达显著减少.

结论:

*MCDBD-PG-NOW是一种控制腹病原体的有希望的方法.
* 通过破坏关键的感染机制,治疗有效地减少了细菌的生存和毒性.
*由MCDBD-PG-NOW生成的RNA显示了预防腹感染的潜力.

关键词:

影响者是影响者.环境污染物的环境污染物.血生成的氧化水 T3SS (第三类分泌系统)这是VBNC的VBNC.在水中传播的致病性细菌.

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