通过蛋白质和转录基因反应丰富的Listeria monocytogenes在滞后阶段的洞察力
Jasper W Bannenberg1, Sjef Boeren2, Marcel H Zwietering1
1Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands.
Food research international (Ottawa, Ont.)
|December 21, 2023
概括
了解Listeria monocytogenes在食品丰富过程中的滞后阶段是关键. 这项研究使用转录组学和蛋白质组学来揭示生理变化,发现补充丰富并没有缩短滞后时间.
科学领域:
- 食品微生物学 食品微生物学
- 细菌生理学 细菌生理学
- 病原体检测检测 病原体检测
背景情况:
- 丰富步骤对于检测食物传播的病原体,如Listeria monocytogenes,至关重要.
- 了解缩过程中的滞后阶段对于改进检测方法至关重要.
- 关于Listeria monocytogenes在丰富过程中的生理动力学知识有限.
研究的目的:
- 研究Listeria monocytogenes在缩的滞后阶段的生理过程.
- 确定影响滞后期持续时间和细菌恢复的因素.
- 评估补充丰富的有效性,以缩短滞后时间.
主要方法:
- 使用了组合的转录组和蛋白组分析.
- 李斯特菌单细胞菌细胞在半弗雷泽丰富 (HFB) 中进行培养.
- 分析了先前有热应激史的细胞.
主要成果:
- 运动基因/蛋白质下调;金属吸收,复苏因子,排泄和氧化应激蛋白质上调.
- 热应激细胞显示出调节的DNA修复,蛋白质重新折叠,细胞壁修复和运输蛋白.
- 补充或使用过的HFB并没有显著减少滞后期的持续时间.
- 在使用过的HFB中进行丰富,最终的细胞度与新鲜HFB相似.
结论:
- 这项研究提供了关于Listeria monocytogenes在缩中的滞后阶段蛋白质组变化的见解.
- 补充半弗雷泽缩不是改善当前缩方法的最佳策略.
- 丰富本身似乎并没有缺乏对细菌生长的关键基质.
相关概念视频
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
01:23Stringent Response in E. coli
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...