Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromatin Packaging01:32

Chromatin Packaging

16.7K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
16.7K
DNA Packaging00:58

DNA Packaging

102.6K
Overview
102.6K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Heterochromatin02:38

Heterochromatin

13.1K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
13.1K
Euchromatin01:01

Euchromatin

6.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.9K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.5K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Comparison of the Efficacy of Ciprofol and Propofol for Rapid Sequence Induction and Intubation in Elective Non-Cardiac Surgery: A Prospective, Randomized, Non-Inferiority Trial.

Drug design, development and therapy·2026
Same author

Immune cell phenotypes in the context of diabetic nephropathy: A Mendelian randomization analysis.

Medicine·2026
Same author

A primordial synaptotagmin-independent function of complexin in regulated exocytosis.

Nature communications·2026
Same author

Morning anticipated leisure pleasure and planned activity type jointly predict daily physical activity: An ecological momentary assessment study.

Psychology of sport and exercise·2026
Same author

A systematic review and meta-analysis of the efficacy of exercise therapy for the rehabilitation of rotator cuff-related shoulder pain: a subgroup analysis of racquet sport athletes.

Frontiers in bioengineering and biotechnology·2026
Same author

Development of a new dispersive liquid-liquid microextraction/UPLC method for determining bisphenols and estrogens in water based on Fe<sub>3</sub>O<sub>4</sub> nanoparticle-enhanced magnetic deep eutectic solvent.

Analytical and bioanalytical chemistry·2026

相关实验视频

Updated: Jul 7, 2025

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.0K

在小鼠精子中重新检查染色素包装.

Qiangzong Yin1, Chih-Hsiang Yang1, Olga S Strelkova1

  • 1Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605, USA.

Genome research
|December 21, 2023
PubMed
概括

之前对哺乳动物精子染色质的研究可能是有缺陷的,因为无细胞DNA受到污染. 新的方法揭示了一个独特的精子染色质状态,突出显示了对基因组组织的不完全理解.

更多相关视频

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.5K

相关实验视频

Last Updated: Jul 7, 2025

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.0K
Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

15.5K

科学领域:

  • 生殖生物学 生殖生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因组学就是基因组学.

背景情况:

  • 哺乳动物的精子拥有独特的,高度紧的基因组,对于受精和早期胚胎发育至关重要.
  • 之前的全基因组研究报告了精子染色质可访问性,核细胞定位,基因组修饰和折叠模式.

研究的目的:

  • 根据潜在的无细胞染色质污染,重新评估现有的全基因组精子染色质数据.
  • 为研究精子染色体结构及其在早期胚胎基因编程中的作用建立准确的方法.

主要方法:

  • 在标准溶解条件下复制以前的全基因组精子染色质调查.
  • 在去除无细胞DNA和优化溶解后,应用ATAC-seq (使用测序测定转化酶可访问染色体) .
  • 分析基因组修饰和染色体折叠模式.

主要成果:

  • 之前的精子染色质谱被重复了,这表明它们主要反映了污染的无细胞染色质.
  • 优化的溶解和无细胞DNA去除揭示了一个独特的精子染色质状态.
  • ATAC-seq确定了与早期发育和转录控制相关的开放基因组位置.
  • 基因组修饰和染色体折叠数据进一步支持先前研究中的污染,尽管技术挑战仍然存在.

结论:

  • 目前对哺乳动物精子染色质的全基因组调查受到无细胞DNA污染的重大影响.
  • 精子基因组组织的准确表征需要严格清除污染物和适当的溶解.
  • 目前对哺乳动物精子染色素包装的理解仍然不完整,需要进一步的研究.