m6A阅读器YTHDC2通过破坏MRPL12的稳定性来抑制肺腺癌瘤的发生
Yingchuan Sun1, Yanyan Liu2, Pengyuan Wang3
1Department of Oncology, Xuchang Central Hospital, No. 666, Wenxuan Street, Dongcheng District, Xuchang, 461000, China. 17703748953@163.com.
Molecular biotechnology
|December 22, 2023
概括
通过减少MRPL12表达,m6A阅读器YTHDC2可以抑制肺腺癌. 过度表达YTHDC2可以抑制瘤生长,入侵和迁移,同时促进亡.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- N6-甲基氨酸 (m6A) 是一种普遍存在的RNA修饰,对细胞过程至关重要.
- 异常的m6A修饰与各种癌症有关,包括肺腺癌 (LUAD).
- YTHDC2是一种m6A读者蛋白,在RNA代谢和基因调节中具有已知的作用.
研究的目的:
- 为了研究YTHDC2在LUAD瘤发生中的功能和机制.
- 为了阐明YTHDC2,MRPL12和LUAD进展之间的关系.
- 为了确定在LUAD中准YTHDC2的治疗潜力.
主要方法:
- 定量实时PCR,西式涂抹和免疫组织化学用于基因/蛋白质表达分析.
- 在体外测试 (EdU,殖民地形成,流细胞计,transwell) 来评估细胞增殖,细胞亡,入侵和迁移.
- 在体内小鼠肺腺癌模型和甲基化RNA免疫沉 (MeRIP) 试验.
主要成果:
- 在LUAD组织和细胞中,YTHDC2的下调.
- 过度表达YTHDC2抑制了LUAD细胞的增殖,入侵和迁移,并诱导了亡.
- YTHDC2破坏了m6A修饰的MRPL12mRNA的稳定,导致MRPL12的表达减少.
- 在LUAD中,MRPL12被上调,其沉默抑制了瘤的生长和移动性.
- 通过降低MRPL12.2的调节,YTHDC2在体内抑制了LUAD瘤的生长.
结论:
- 在LUAD中,YTHDC2充当瘤抑制剂.
- YTHDC2通过以m6A依赖的方式破坏MRPL12mRNA的稳定性来抑制LUAD瘤发生.
- 针对YTHDC2或调节MRPL12水平可能为LUAD提供治疗策略.
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Loss of Tumor Suppressor Gene Functions
4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
Mismatch Repair
4.9K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.9K


