循环-NUP98通过通过miR-188-3pp调节CBX1促进肺腺癌的发展
Jie Mei1, Jing Zuo1, Jiazhuan Mei2
1Department of Clinical Laboratory, The Fifth Clinical Medical College of Henan University of Chinese Medicine (Zhengzhou People's Hospital), Zhengzhou, 450000, China.
Biochemical genetics
|December 22, 2023
概括
循环RNA NUP98 (circ-NUP98) 促进肺腺癌 (LUAD) 的发展,通过菌微RNA-188-3p来调节染色体同源1 (CBX1). 下调circ-NUP98可以抑制LUAD的进展和瘤的生长.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 肺腺癌 (LUAD) 是癌症死亡的主要原因.
- 循环RNAs (circRNAs) 越来越多地被认为是它们在瘤发生中的作用.
- 在LUAD进展中circ-NUP98的特定功能仍然在很大程度上没有特征.
研究的目的:
- 研究circ-NUP98在LUAD中的作用和分子机制.
- 探索circ-NUP98作为LUAD诊断标记物的潜力.
主要方法:
- 使用RT-qPCR对circ-NUP98,microRNA-188-3p (miR-188-3p) 和染色盒同源1 (CBX1) 的量化.
- 通过CCK-8,EDU,流细胞计,伤口愈合和Transwell检测评估LUAD细胞的增殖,亡,迁移和入侵.
- 使用异种移植瘤模型进行体内评估,并通过双化酶报告者和RIP测定分析分子相互作用.
主要成果:
- 在LUAD组织和细胞中,Circ-NUP98和CBX1表达升高,而miR-188-3p降低.
- 对circ-NUP98的下调抑制了LUAD细胞的增殖,迁移,入侵和氧化应激,同时促进了细胞亡.
- Circ-NUP98作为miR-188-3p的分子海绵,从而增加CBX1的表达,并在体内抑制LUAD瘤生长.
结论:
- Circ-NUP98通过miR-188-3p/CBX1信号轴促进LUAD恶性进展.
- Circ-NUP98代表了一个潜在的治疗标,也是早期LUAD诊断的一个有前途的生物标志物.
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