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从不到100个活细菌中量化分析物的皮克莫尔:一种新的方法,使用缓冲水凝作为电化学细胞

Bronson Samel-Garloff¹, Subir Goswami¹, Ankan Ghosh¹

¹Department of Chemistry, Oregon State University, Corvallis OR.

Electrochimica acta

|December 22, 2023

概括

这项研究引入了一种新的水凝电化学细胞,用于精确测量由小细菌群产生的过氧化 (H2O2). 该平台可以从最小的生物样本中量化敏感的代谢物,克服以前的分析局限性.

科学领域:

生物化学生物化学
分析化学分析化学
微生物学微生物学

背景情况:

微环境变化显著影响细菌生物膜生态.
从小数量的细菌中定量分析物提出了分析方面的挑战.

研究的目的:

开发一种基于微升水凝的新型电化学电池,用于精确测量过氧化 (H2O2).
为了能够量化由不到100个细菌细胞产生的H2O2,特别是Streptococcus sanguinis*.

主要方法:

在水凝聚合物中加入一个形态素部分,以在生物pH下创建一个受控的微环境.
开发一种用于计算水凝缓冲容量的新方法.
使用涂层在缓冲水凝中的H2O2传感器,在广泛的pH范围 (7.0-3.0) 中进行稳定的测量.

主要成果:

水凝在7.2-6.2.2的pH范围内表现出0.257±0.135的缓冲容量.
H2O2传感器的灵敏度从pH值7.0到3.0保持稳定,允许独立于细菌酸生产的测量.
该平台成功地从不到100个*S. sanguinis*细胞中测量了低至22.7 ± 3.5 pmol的H2O2.

结论:

开发的水凝电化学细胞平台适用于从亚毫克生物样本中量化代谢物.
这种新的平台可以从微小的细菌数量中实现以前无法实现的H2O2测量.
该系统显示了对原始生物膜样本直接分析的潜力,预处理最小.

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