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基于欧米克数据,对参与肺动脉高血压病变的单细胞相关途径的鉴定

Caiming Zhong^1,2, Yachen Si³, Huanhuan Yang¹

¹Department of Respiratory and Critical Care Medicine, Shanghai Changzheng Hospital Naval Medical University Shanghai China.

Pulmonary circulation

|December 22, 2023

概括

肺动脉高血压 (PAH) 涉及免疫变化,特别是减少单细胞透. 像ICAM-1和ITGAL-ITGB2这样的单细胞相关途径与PAH病变发生有关.

科学领域:

免疫学免疫学免疫学
心血管研究研究心血管研究
基因组学就是基因组学.

背景情况:

肺动脉高血压 (PAH) 是一种慢性,无法治愈的疾病,导致心力衰竭.
免疫微环境被认为是PAH发展的关键因素.
了解免疫细胞的作用是解开PAH病变的关键.

研究的目的:

研究免疫细胞透在肺动脉高血压 (PAH) 病变发生过程中的作用.
根据免疫细胞概况,识别PAH潜在的诊断生物标志物.

主要方法:

来自微阵列数据的差异表达基因的分析,富含与免疫相关的途径.
使用ssGSEA和CIBERSORT对GEO数据库微阵列数据进行免疫细胞透评估.
单细胞RNA测序 (scRNA-seq) 用于详细的细胞特异性作用和细胞间通信.
接收器操作特征 (ROC) 曲线和最小绝对收缩和选择操作员 (LASSO) 用于生物标志物发现.

主要成果:

在PAH患者中通过微阵列和scRNA-seq数据观察到单细胞透的显著下调.
在PAH中改变了细胞间通信网络,突出显示ITGAL-ITGB2和ICAM信号通路.
ITGAM和ICAM2证明了PAH的显著诊断价值.

结论:

单细胞变化代表了肺动脉高血压 (PAH) 的关键免疫特征.
单细胞相关的ICAM-1和ITGAL-ITGB2信号通路可能参与PAH的发病.
作为PAH的诊断生物标志物,ITGAM和ICAM2显示出有希望的结果.

关键词:

在ICAM‐1信号通道中.在ITGAL-ITGB2信号通道中.免疫透免疫透是什么?单细胞 (Monocytes) 是一种单细胞.肺动脉高血压肺动脉高血压

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