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在甲状腺相关轨道病变的轨道脂肪/连接组织中差异表达的基因

Yan Wang¹, Yanqiu Liu¹, Jiping Cai¹

¹Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.

|December 22, 2023

概括

甲状腺相关的轨道病 (TAO) 涉及轨道脂肪的遗传差异,炎症基因增加和分化/发育基因减少. 这种分子洞察力可能解释了TAO.

科学领域:

眼科医生眼科眼科
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

甲状腺相关的轨道病 (TAO) 是一种自身免疫性甲状腺疾病,导致亡,双眼视,视力丧失.
了解TAO中的轨道脂肪生成对于阐明疾病机制至关重要.

研究的目的:

描述TAO患者和对照的轨道脂肪/连接组织中的分子差异.
确定涉及TAO病变发生的关键基因和途径.

主要方法:

RNA测序 (RNA-seq) 用于分析轨道脂肪/连接组织中的基因表达.
生物信息分析包括GO,KEGG,GSEA和PPI网络建设.
使用定量实时PCR (qRT-PCR) 验证差异表达基因 (DEGs).

主要成果:

在TAO和对照组织之间确定了183个DEG (114个上调,69个下调).
免疫和炎症反应基因 (例如,CP,ALPL,AGT) 在TAO中被上调.
在TAO中观察到的差异化和发育基因 (例如,MAB21L1,PIK3C2G,CLVS2,RSPO1) 的下调.

结论:

在轨道脂肪连接组织中,TAO表现出明显的遗传特征.
上调的炎症反应与TAO的临床特征相关,如眼胀和外眼.
下调的基因表明TAO的差异化,氧化应激和发育途径的失调.

关键词:

不同表达的基因.高通量测序的高通量测序炎症炎症是一种炎症.甲状腺相关的眼科疾病.它们是mRNARNA.

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