增强的生长和代谢物产量来自新型Porphyridium sp菌株
Latifa Tounsi1,2, Hajer Ben Hlima1, Hana Derbel1
1Laboratory of Enzymatic Engineering and Microbiology, Algae Biotechnology Team. National Engineering School of Sfax, University of Sfax, Sfax, Tunisia.
Bioengineered
|December 22, 2023
概括
这项研究确定了突尼斯的一种新的Porphyridium微藻菌株. 确定了生物质,色素,蛋白质,脂质和外聚糖类生产的最佳培养介质,这对于工业应用至关重要.
科学领域:
- 海洋生物技术 海洋生物技术
- 微藻类生理学 微藻类生理学
- 物理学的生理学
背景情况:
- 微藻提供了具有工业潜力的多样化代谢物.
- 微藻的分类学多样性仍然未被充分探索.
- 一种新的Porphyridium菌株从突尼斯海岸被隔离出来.
研究的目的:
- 使用18S rRNA基因识别新发现的微藻菌株.
- 调查Porphyridium sp.的生长和代谢物积累情况. 在不同的媒体条件下.
- 为了优化生物质,颜料,蛋白质,脂质和外聚糖的栽培.
主要方法:
- 18S rRNA基因测序用于分类识别.
- 培养Porphyridium sp.的种植方式 在PM,F/2和Hemerick媒体上超过52天.
- 生物质,叶绿素a,胡卜素, phycoerythrin,蛋白质,粉,脂质和外聚糖的量化.
主要成果:
- Pm介质产生了最高的生物质 (2 × 10^7细胞/毫升),增长率 (μ) 为0.081天-1.1.
- F/2介质最大化了叶绿素a,胡卜素,植物和蛋白质含量.
- 黑梅里克介质对脂质 (2.23%DW) 和外聚糖类 (5.41 pg/细胞) 生产是最佳的.
结论:
- 这种已识别的菌株属于Porphyridium属.
- 不同的培养介质显著影响Porphyridium sp. 增长和代谢物概况.
- 优化介质选择是从工业用途的这种微藻菌株中准代谢物生产的关键.
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