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Updated: Jul 7, 2025

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

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在Vibrio natriegens中通过使用CRISPR相关的转基因酶的LoxP介导进化进行染色体重组和修饰

Jiaqi Xu1,2, Yijie Sun2, Jianping Wu1,2

  • 1Institute for Intelligent Bio/Chem Manufacturing, ZJU-Hangzhou Global Scientific and Technological Innovation Centre, Hangzhou, China.

Biotechnology and bioengineering
|December 22, 2023
PubMed
概括

研究人员使用CRISPR相关的转体酶 (CAST) 来有效地将LoxP位点插入Vibrio natriegens. 这使得通过LoxP介导进化 (CRaMbLE) 实现染色体重排和修改,确定了导致快速生长的关键片段.

关键词:
与CRISPR相关的转基因酶.通过LoxP介导的进化进行染色体重组和修改.维布里奥纳特里根斯 (Vibrio natriegens) 是一种天然物质.快速增长 快速增长 快速增长 快速增长

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Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach11:12

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