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采集和运送植物样本用于多原子分析和植物卫生评估

Esra Karakas¹, Paola Ferrante², Roland Schafleitner³

¹Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.

Current protocols

|December 22, 2023

概括

本研究介绍了用于基因组学和代谢学两方面的收集,运输和处理植物样本的统一协议. 这些方法可确保从作物植物获得高质量的多组数据,即使在长途运输后也是如此.

科学领域:

植物科学植物科学
基因组学就是基因组学.
代谢学代谢学代谢学

背景情况:

精确的新陈代谢需要在植物样本中灭水解酶.
标准的DNA保存方法通常与代谢物保存相兼容.

研究的目的:

描述结合植物基因组学和代谢学的综合性协议.
为大规模作物研究从单个样本收集多主题数据.
为新鲜冷和冷干燥的植物材料提供可适应的协议,包括长途运输.

主要方法:

采样,植物卫生运输和加工的详细程序.
准备冷干燥和新鲜冷植物材料的代谢学协议.
用于各种基因造型应用的DNA提取和质量控制方法.
用于代谢分析的代谢物提取和样本运输的特殊协议.

主要成果:

从单个植物样本进行同时DNA和代谢物分析的既定协议.
在长途运输过程中保存样品完整性的验证方法.
适用于冷干燥和新鲜冷植物组织的可适应性协议.
在大型实验设置中成功生成多omics数据集.

结论:

统一的样本准备策略促进了综合基因组学和代谢学研究.
描述的协议支持从作物植物中获得强大的多学科数据.
这些方法对于准确分析植物的遗传和代谢资料至关重要.

关键词:

DNA 条形码编码基因型定型是指基因型定型.代谢生物组的代谢生物组植物检疫证书植物检疫证书采样采样采样采样

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