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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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相关实验视频

Updated: Jul 7, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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用表达和序列信息来进行皮肤表达 lncRNA 的大规模功能推断.

Matthew T Patrick1, Sutharzan Sreeskandarajan1,2, Alanna Shefler1

  • 1Department of Dermatology, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA.

JCI insight
|December 22, 2023
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概括

长非编码RNAs (lncRNAs) 在炎症性皮肤疾病中至关重要. 这项研究确定了特定的lncRNA及其DNA结合域,揭示了它们在皮肤免疫和疾病中的作用.

关键词:
生物信息学是一种生物信息学.皮肤病学 皮肤病学遗传变异是一种遗传变异.遗传学 是一个遗传学.皮肤 皮肤 皮肤 皮肤

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科学领域:

  • 基因组学就是基因组学.
  • 免疫学 免疫学 免疫学
  • 皮肤病学 皮肤病学

背景情况:

  • 长非编码RNAs (lncRNAs) 调节基因表达,并与炎症性皮肤疾病有关.
  • 由于组织的特异性和低表达,了解皮肤中的lncRNA功能是有限的.

研究的目的:

  • 在炎症性皮肤疾病中全面分析lncRNA表达和功能.
  • 确定特定的lncRNA及其参与皮肤免疫的DNA结合域.

主要方法:

  • 编制了18517个lncRNA的数据集,并分析了834个RNA-Seq样本的表达特征.
  • 采用随机森林模型来预测生物功能并识别DNA结合域 (DBD).
  • 使用单细胞和空间测序来验证细胞类型的特异性.

主要成果:

  • 确定了39个lncRNA的显著DBD,其中位数AUROC为0.79.
  • 发现了与巨细胞调节基因F13A1相关的lncRNA (G18244),并确定了DBD.
  • 发现 lncRNAs AC090198.1 和 AC005332.6 与亚托皮炎严重程度 (SCORAD) 有负相关性.

结论:

  • lncRNAs在皮肤中起着重要的免疫作用.
  • 这项研究为在炎症性皮肤疾病研究中优先考虑 lncRNAs 提供了框架.
  • 已识别的lncRNAs为皮肤炎症条件提供了潜在的治疗点.