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表面功能化和大肠杆菌检测使用表面增强的拉曼光谱,由功能性有机聚合物/金纳米基微流体芯片驱动

Hugo Cortes-Cano¹, Lilian Iraís Olvera², Emilia M Méndez-Aguilar³

¹Dirección de Ciencia, Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Parque Tecnológico Querétaro S/N, Sanfandila, Querétaro 76703, Pedro Escobedo, Mexico.

|December 22, 2023

概括

本研究介绍了一种新的微流体装置,用于使用表面增强拉曼光谱 (SERS) 监测表面过程. 该原型可在使用最小样本的情况下对污染物和微生物进行敏感检测.

科学领域:

材料科学材料科学材料科学
分析化学分析化学
生物技术是生物技术.

背景情况:

环境污染和有限的样本量对实时地表过程监测构成了挑战.
表面增强拉曼光谱 (SERS) 提供高灵敏度,但需要优化基板.
聚合物微流体设备对集成分析系统具有吸引力.

研究的目的:

开发一种微流体原型,用于基于SERS的表面过程监测.
为试剂和样品创造一个没有污染的环境.
调查检测微生物和监测随时间变化的化学变化的潜力.

主要方法:

用功能性有机聚合物 (FOP) 膜制造一个聚甲基酸 (PMMA) 微流体装置.
在FOP上沉积金 (Au) 纳米薄膜以增强SERS.
COMSOL 多物理模拟用于分析电场分布和增强因子.
使用紫外线可见光谱,拉曼光谱和X射线光电子光谱 (XPS) 进行了表征.
使用拉曼显微镜对囊胺和大肠杆菌 (E. coli) 溶液进行测试.

主要成果:

确认Au膜形态对于SERS效应至关重要.
在FOP表面上实现了高达8.8 × 10^5的高功率增强系数.
证明了化学化合物和大肠杆菌的成功检测和时间监测.
证实了氨基组的表面功能化.

结论:

开发的微流体SERS原型有效监测表面过程并检测微生物.
该设备尽量减少试剂的使用,环境污染和测量干扰.
这项技术显示出在生物和化学应用中及时评估表面的前景.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.功能性的有机聚合物有机聚合物.微流体原型的原型微生物检测微生物检测功率增强因素是功率增强因素.模拟模拟是指一个模拟模拟.表面功能化的功能化.表面增强的拉曼光谱学

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