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在环境和宿主温度下对Yersinia enterocolitica的DNA甲基化进行比较
Dustin J Van Hofwegen1, Carolyn J Hovde1, Scott A Minnich1
1Department of Animal Veterinary and Food Science, University of Idaho, Moscow, ID 83843, USA.
Epigenomes
|December 22, 2023
概括
差异性DNA甲基化在温度变化期间调节Yersinia enterocolitica中的基因表达. 这种表观遗传机制影响细菌适应宿主环境,影响毒性和运动性.
科学领域:
- 微生物学 微生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细菌病原体的产生
背景情况:
- 病原性细菌适应基因表达环境线索,以殖民宿主.
- 在温度升高时,Yersinia enterocolitica表现出快速抑制鞭子合成和诱导III型分泌系统,但在温度降低时,运动恢复速度较慢.
研究的目的:
- 为了研究差异性DNA甲基化在Yersinia enterocolitica中温度调节的基因表达中的作用.
- 为了确定与温度依赖的基因表达变化相关的特定DNA甲基化模式.
主要方法:
- 单分子实时测序 (SMRT) 用于分析22°C和37°C生长的Yersinia enterocolitica的DNA甲基化.
- 脉冲间持续时间比被用作DNA甲基化分析的动力度量.
- 分析的重点是DNA腺甲基酶 (Dam) 和Dcm位点.
主要成果:
- 虽然所有YenI限制部位都完全甲基化,但在27,118个DNA腺甲基酶 (Dam) 部位中,42个显示了两种温度之间的甲基化差异.
- 17个位始终没有甲基化,主要位于复制终点附近.
- 差异甲基化的Dam位点被发现在编码转录调节器和循环-di-GMP二酶的基因的促进子区域.
结论:
- 不同的DNA甲基化,特别是在Dam位点,在Yersinia enterocolitica的温度调节的基因表达中起作用.
- 表观遗传修饰通过调节毒性因子和调节基因的表达,有助于细菌的适应.
- 这些发现表明,DNA甲基化,细胞循环和观察到的温度依赖的生理变化之间存在联系.
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