准Cx43以减少压力进展的严重程度
Milton Sheng Yi Kwek1,2, Moogaambikai Thangaveloo1,2, Leigh E Madden1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11, Mandalay Road, Singapore 308232, Singapore.
研究人员发现,阻断间隙结蛋白Cx43可以防止压力的恶化. 这种有针对性的方法可以阻止第一阶段的压力进展到更严重的阶段.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 皮肤中反复发生的缺血-再输液损伤会导致压力.
- 第1阶段的压力包括表皮Cx43升高和炎症.
- 进展到第二阶段的压力包括表皮损伤.
研究的目的:
- 调查Cx43在压力的形成和进展中的作用.
- 评估Cx43反感性寡核酸在预防压力发作的治疗潜力.
主要方法:
- 在小鼠中使用磁铁诱导缺血-反损伤.
- 评估表皮Cx43表达,炎症细胞透,以及死和死的标志物.
- 用Cx43反感性寡核酸剂来评估它们对压力进展的影响.
主要成果:
- 单次缺血-再输液事件增加了表皮Cx43,中性粒细胞,HMGB1和RIP3.3.
- 在一次侮辱后,Cx43反感治疗减少了表皮Cx43,中性粒细胞,HMGB1和RIP3.
- 在一个双进攻模型中,Cx43反感治疗缓解了炎症,亡,组织损伤和伤口进展.
结论:
- 皮肤上升的Cx43是压力发育期间无菌炎症反应的关键因素.
- 用反感性寡核酸向Cx43,有望防止压力从第一阶段发展到第二阶段.
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