使用EDTA和HEDP作为化剂的内治疗牙的2D和3D侵蚀景观分析:一个高分辨率的微计算断层扫描研究
Parichehr Zarean1, Michael Göllner2, Paridokht Zarean1
1Department of General Pediatric and Adolescent Dentistry, University Center for Dental Medicine Basel UZB, University of Basel, Mattenstrasse 40, 4058 Basel, Switzerland.
Dentistry journal
|December 22, 2023
概括
这项研究使用微CT比较了HEDP和EDTA在内牙灌期间的侵蚀.HEDP的根管侵蚀明显低于EDTA,这表明它是一种更安全的灌剂.
科学领域:
- 牙周内科医院 牙周内科医院 牙周内科医院
- 牙科材料科学 牙科材料科学
- 生物材料是一种生物材料.
背景情况:
- 激活的内牙灌对于废物清除和消毒至关重要.
- 使用EDTA和HEDP等化剂来去除涂抹层.
- 这些剂对根管牙的潜在侵蚀作用需要仔细评估.
研究的目的:
- 量化评估和比较根管牙侵蚀,由激活灌以乙烯基二胺三酸 (EDTA) 和乙烯二酸盐 (HEDP) 引起.
- 使用高分辨率的微型计算机断层扫描 (微型CT) 进行精确的二维和三维侵蚀测量.
主要方法:
- 使用ProTaper Next准备了20个前根通道,并用二化灌.
- 用EDTA (n=10) 或HEDP (n=10) 灌口腔通道,而口腔通道作为控制.
- 微型CT扫描用于测量二维和三维侵蚀深度,随后进行统计分析 (ANOVA和后期测试).
主要成果:
- 两种2D和3D分析都显示了EDTA和HEDP之间侵蚀的显著差异 (p <0.01).
- 与HEDP (20.25, 16.40, 15.96 μm) 相比,EDTA在宫,中间和顶端三分之一 (45.75, 41.79, 32.25 μm) 造成的平均侵蚀深度明显更大.
- 在EDTA组内,在宫和顶部位置 (p = 0.03) 观察到侵蚀的显著差异.
结论:
- 与EDTA相比,HEDP在激活灌中使用时,对根管牙的侵蚀效应明显较小.
- 微CT是量化灌剂诱导侵蚀的有价值工具,适用于评估各种灌方案,特别是在更恶劣的条件下,如内牙再处理.
相关概念视频
EDTA: Chemistry and Properties
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Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
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Effects of EDTA on End-Point Detection Methods
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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Masking and Demasking Agents
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EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
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EDTA: Auxiliary Complexing Reagents
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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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EDTA: Direct, Back-, and Displacement Titration
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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
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EDTA: Indirect and Alkalimetric Titration
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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
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