结肠直肠癌的体质拷贝数变化导致差异表达的ceRNA网络 (ceRNet)
Héctor Herrera-Orozco1,2, Verónica García-Castillo1, Eduardo López-Urrutia1
1Laboratorio de Genómica, FES-Iztacala, Universidad Nacional Autónoma de México. Av. De los Barrios 1, Los Reyes Iztacala, Tlalnepantla 54090, Mexico.
Current issues in molecular biology
|December 22, 2023
概括
大肠直肠癌 (CRC) 涉及染色体不稳定. 这项研究揭示了拷贝数的改变如何影响长非编码RNA (lncRNAs),影响CRC并确定患者生存的关键 lncRNAs.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 大肠直肠癌 (CRC) 是癌症死亡的主要原因.
- 染色体不稳定,导致拷贝数的改变,在CRC中很常见.
- 在长非编码RNA (lncRNA) 调节和CRC表型中拷贝数变化的作用需要进一步研究.
研究的目的:
- 调查结直肠癌中复制数改变区域中差异表达的lncRNAs.
- 构建一个竞争的内源RNA (ceRNA) 网络,以了解 lncRNA介导的基因调节.
- 评估lncRNA表达在CRC患者生存中的临床相关性.
主要方法:
- 利用癌症基因组图谱 (TCGA) 存储库用于结直肠腺癌RNA-seq和副本数量改变数据.
- 对lncRNAs,miRNAs和mRNAs进行了差异表达分析.
- 构建了一个lncRNA-miRNA-mRNA ceRNA网络,并进行KEGG/GO通路和生存分析.
主要成果:
- 鉴定了78个差异表达的lncRNA,410个miRNA和5028个mRNA.
- 建立了一个包含30个lncRNA,19个miRNA和77个mRNA的ceRNA网络,富含致癌和抗致癌途径.
- 发现C5orf64,HOTAIR和RRN3P3 lncRNA表达水平与患者的整体生存时间相关.
结论:
- 位于体质获得的拷贝数改变区域的lncRNAs是结直肠癌中复杂基因调节网络的组成部分.
- 这些lncRNAs在CRC病变发生过程中起着重要作用,并代表潜在的预后生物标志物.
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