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鉴定和JHE 6的功能 具体表达在Bombyx mori丝腺
Xia Zhang1, Jikailang Zhang1, Keli Wu1
1State Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, China.
丝虫中的青春期激素酶6 (Bmjhe6) 调节丝腺发育和蛋白质合成. 它的打击加速了幼虫的生长,并通过调节青少年激素信号来影响丝基因表达.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 青春激素 (JH) 对于昆虫的发育和繁殖至关重要.
- JH调节了Bombyx mori.中的丝腺发育和丝蛋白合成.
- 青春期激素雌激酶 (JHE) 通过降解它来控制JH水平.
研究的目的:
- 在Bombyx mori.中识别和描述JHE家族成员.
- 研究Bmjhe6在丝腺功能和发育中的特定作用.
- 阐明Bmjhe6对荷尔蒙反应的调节机制.
主要方法:
- 使用生物信息学识别JHE家庭成员.
- 半定量和定量实时RT-PCR用于基因表达分析.
- 西方斑点和免疫光用于蛋白质定位.
- 激素诱导实验和RNA干扰 (RNAi) 用于功能分析.
主要成果:
- 确定了9个Bmjhe基因,其中Bmjhe6在丝腺特异性表达,特别是在前部中部丝腺.
- Bmjhe6被分泌到丝腺膜中,并与丝蛋白共同运输.
- Bmjhe6表达被JH模拟上调调节,并被20-hydroxyecdysone抑制.
- Bmjhe6 knockdown加速了幼虫的发育,增加了幼虫和丝腺的体重,破坏了素基因表达,并提高了JH合成途径基因的调节.
结论:
- Bmjhe6是Bombyx mori.中的丝腺生长和丝蛋白合成的关键调节剂.
- Bmjhe6调节青春期激素信号,影响幼虫发育和丝生产.
- 这项研究提供了关于丝虫发育和丝生产的复杂激素调节的见解.
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