在果皮病原体中发现的对醇杀菌剂的剂量依赖性遗传耐药性
Thomas Heaven1,2,3, Andrew D Armitage4, Xiangming Xu1
1National Institute of Agricultural Botany, New Road, East Malling, West Malling, Kent ME19 6BJ, UK.
Journal of fungi (Basel, Switzerland)
|December 22, 2023
概括
果病原体中的真菌醇真菌杀菌剂耐药性是多基因的. 在Venturia的两个定量特征位点 (QTLs) 不等于控制耐药性,特定的基因被确定为诊断的潜在目标.
科学领域:
- 农业科学 农业科学
- 植物病理学 植物病理学
- 遗传学 是一个遗传学.
背景情况:
- 醇杀菌剂对于管理农业和人类健康中的真菌病至关重要.
- 果病原体*Venturia inaequalis*中出现的阿佐尔耐药性威胁着作物生产.
- 现有的耐药性机制,如位突变,不能完全解释敏感性降低.
研究的目的:
- 为了研究多基因对Tebuconazole耐药性的遗传基础,在*Venturia inaequalis*.
- 为了确定与真菌杀菌剂耐药性相关的定量特征位点 (QTLs).
- 在已识别的QTL区域内探索潜在的耐药性基因候选者.
主要方法:
- 通过测序 (GBS) 的基因型鉴定被用来绘制QTLs.
- 基于人群的分析被用来研究剂量依赖性耐药性.
- 在QTL区域内检查候选基因的基因变异.
主要成果:
- 两个主要的QTLs显著促进了特布可纳耐药性.
- 链接组七的QTL解释了ED50的65%变化,并影响了较低剂量耐药性 (ED10).
- 第二个QTL在链接组一解释了ED10的34%的变化,但不是在更高的剂量.
结论:
- *Venturia inaequalis*的真菌杀菌剂耐药性是由多个基因控制的,其影响取决于剂量.
- ATP结合盒和主要促进者超级家族的转运基因与抵抗有关.
- 识别这些耐药基因可以帮助开发用于疾病管理的分子诊断.
关键词:
生物的生物类动物剂量响应的剂量反应基因组 基因组是基因组的组成部分.通过测序进行基因型鉴定.链接地图 链接地图植物病原体的病原体多基因的多基因.定量特征位点 (QTLs) 是指数量特征位点.单核酸多态性 (SNP) 是指单核酸多态性.这种药物是tebuconazole.更多相关视频
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