相关实验视频

两种pksCT基因转录的代谢调节在Monascus ruber中影响氨酸生物合成

Yi He^1,2, Lisha Zhu^1,2, Xingxing Dong¹

¹National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

Journal of fungi (Basel, Switzerland)

|December 22, 2023

概括

这项研究揭示了pksCT基因的替代拼接如何调节Monascus真菌中的氨酸生产. 沉默pksCT转录显著降低氨酸,影响新陈代谢途径,并提供新的研究途径.

科学领域:

菌类学菌类学是指菌类学.
生物化学生物化学
分子生物学分子生物学

背景情况:

来自Monascus的有毒代谢物Citrinin (CIT) 限制了产品的使用.
CIT生物合成是由pksCT基因和其他遗传因素控制的.

研究的目的:

研究pksCTα和pksCTβ转录在CIT生物合成中的调控作用.
阐明蒙纳斯库斯中CIT生产和替代拼接的机制.

主要方法:

利用头RNA (ihpRNA) 干扰来使pksCT转录保持沉默.
采用化学生物学和转录组分析来评估对CIT生产和基因表达的影响.

主要成果:

制pksCTα和pksCT (α + β) 降低了CIT产量,分别从7.2微克/毫升降至3.8微克/毫升和0.08微克/毫升.
在CIT生物合成集群中观察到关键基因 (mrl3,mrl5,mrr1,mrr5) 的下调.
显著抑制TCA循环和糖解酶,减少前体的可用性 (乙-CoA,马洛尼尔-CoA).

结论:

pksCT替代拼接是Monascus中CIT生物合成的关键调节器.
减少CIT影响碳水化合物,氨基酸和脂质代谢.
这些发现为了解CIT生产和开发Monascus研究策略提供了基础.

关键词:

莫纳斯库斯 (Monascus ruber) 是一个红色的植物.这是一种RNA干扰.另一个替代拼接方法是拼接.氨酸 (citrinin) 是一种氨酸.葡萄糖溶解是什么脂质新陈代谢脂质新陈代谢

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