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双RNA-Seq揭示了温度介导的基因重编程和葡萄藤和Lasiodiplodia theobromae之间的分子交叉声

Junbo Peng¹, Yonghua Li¹, Qikai Xing¹

¹Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Journal of fungi (Basel, Switzerland)

|December 22, 2023

概括

气候变化带来的高温加速了植物疾病的发展. 这项研究揭示了葡萄藤和病原体*Lasiodiplodia theobromae*如何根据温度波动重新编程基因表达,揭示了感染期间复杂的共同转录网络.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
气候变化对农业的影响

背景情况:

全球气温上升和气候变化加剧了世界各地的植物疾病.
了解热应激下的植物病原体相互作用对于疾病管理至关重要.
温度介导的植物免疫力和病原体毒性的机制在很大程度上是未知的.

研究的目的:

为了研究高温对葡萄藤癌症发展的影响.
量化葡萄藤和Lasiodiplodia theobromae中的温度驱动的基因转录变化.
阐明温度调节的植物病原体相互作用背后的分子机制.

主要方法:

使用双RNA测序 (RNA-seq) 方法同时分析葡萄藤和Lasiodiplodia theobromae中的基因表达.
量化转录的重编程,以应对不同的温度条件.
在两种生物体中确定了相关的基因模块和温度敏感基因.

主要成果:

葡萄藤和L. theobromae都在不同的温度下表现出显著的转录组变化.
鉴定了26个葡萄和17个真菌基因模块与生物之间的相关性,表明感染期间发生了广泛的重编程.
在宿主和病原体中发现了参与压力反应,新陈代谢和信号通路的温度敏感基因.

结论:

葡萄藤和L. theobromae之间存在复杂的共同转录网络,其调节因温度变化而改变.
温度波动在疾病发展过程中激活了两种生物体的复杂信号通路.
这项研究为温度影响植物疾病动态的分子基础提供了新的见解.

关键词:

拉西奥迪普洛迪亚是什么意思拉西奥迪普洛迪亚是什么意思双重RNA-seqq 的情况.葡萄藤是一种葡萄.温度的温度的温度的温度的温度转录重编程重编程

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