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技术报告:Nightingale Health的H-NMR代谢学平台的不同量化版本之间的全面比较

Daniele Bizzarri^1,2,3, Marcel J T Reinders^2,3, Marian Beekman¹

¹Molecular Epidemiology, Department of Biomedical Data Science, Leiden University Medical Center, 2333 ZC Leiden, The Netherlands.

|December 22, 2023

概括

奈丁盖尔健康最新的NMR代谢量化与临床标准保持一致,但可能会影响研究可复制性. 研究人员在使用不同平台版本时必须谨慎使用,以确保数据的一致性.

科学领域:

生物化学生物化学
临床化学临床化学
生物信息学是一种生物信息学.

背景情况:

质子核磁共振 (H-NMR) 代谢学对于健康和疾病监测至关重要.
作为H-NMR代谢组分的关键供应商之一的Nightingale Health更新了其量化策略,以满足临床标准.
量化更新可能会影响纵向研究的向后可复制性.

研究的目的:

为了比较Nightingale Health的H-NMR代谢学数据量化版本 (2014/2016与2020).
评估量化更新对分析剂一致性和多分析剂评分的影响.
评估以前确立的死亡率关联的可复制性.

主要方法:

利用了BBMRI-NL联盟的数据 (约28,000个样本,28个队列).
比较原始 (2014/2016) 和重新定量 (2020) 的Nightingale健康数据来自相同的NMR光谱.
使用斯皮尔曼相关性 (ρ) 分析了分析物对应性,并评估了多分析物得分 (MetaboAge,MetaboHealth).

主要成果:

对于大多数分析品 (73/222与 ρ > 0.9) 观察到高一致性.
五种分析样本 (乙乙酸,LDL-L,和脂肪酸,S-HDL-C,斯芬戈米林) 显示连续较低的相关性 (ρ < 0.7).
MetaboHealth复制得很好;MetaboAge由于停止分析而需要重新培训,但这两项得分都保留了死亡率关联.

结论:

奈丁盖尔健康最新的量化策略显示了很高的一致性,但需要仔细考虑特定的分析物.
研究人员应该谨慎地混合不同的平台版本,以避免数据不一致 (例如单位,已停产的分析物).
虽然更新的数据总结了关键发现,但为了纵向可重复性,可能需要重新训练分数.

关键词:

对于NMR的代谢组分.流行病学流行病学多变量风险模型的多变量风险模型诺丁加尔健康诺丁加尔健康重定量化的重新定量化.

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