将橄叶富含多醇的提取物封装在聚合物微粒中,以提高其肠道透性
Maria Camilla Bergonzi1, Chiara De Stefani1, Marzia Vasarri1,2
1Department of Chemistry Ugo Schiff, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Florence, Italy.
Nanomaterials (Basel, Switzerland)
|December 22, 2023
概括
聚合物微粒增强了橄叶多的肠道吸收,包括橄蛋白. 这种配方改善了聚醇的输送和生物可用性,用于潜在的治疗应用.
科学领域:
- 药理学和制药科学 药理学和制药科学
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
背景情况:
- 橄叶提取物 (OLE) 富含多,如oleuropein,以健康益处而闻名,但由于肠道透性差,受到限制.
- 开发有效的输送系统对于提高OLE多的生物可用性和治疗效果至关重要.
- 聚合物微粒提供了一个有前途的纳米载体方法,用于提高疏水化合物的溶解性和吸收性.
研究的目的:
- 开发和表征稳定的聚合物微粒,以改善Olea europaea L.叶子提取物的肠道输送.
- 为了评估发育的小粒细胞的封装效率,物理化学性质和体外释放动力学.
- 使用体外模型评估聚合物微粒对多透性的影响.
主要方法:
- 使用前配方研究和冷干燥来创建稳定的细胞配方.
- 特性包括颗粒大小,多分散度指数 (PdI) 和封装效率.
- 在试验室中,使用并行人工膜透性试验 (PAMPA) 和Caco-2细胞单层进行了透性评估.
主要成果:
- 一种优化的微粒配方 (15 mg/mL OLE,Pluronic L121,Pluronic F68,TPGS) 实现了小颗粒大小 (14.21 nm) 和高封装效率 (66.21%).
- 聚合物微粒表现出良好的稳定性,并控制了多的体外释放.
- 两种PAMPA和Caco-2测定都证实了通过聚合物微粒显著增强的多透.
结论:
- 聚合物微粒有效地封装并改善Olea europaea L.叶子多的肠道透性.
- 开发出来的菌根系统显示出有潜力提高橄叶提取物成分的生物可用性.
- 这种方法提供了一种可行的策略,用于输送多和其他透性较差的生物活性化合物.
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