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在Plutella xylostella中Dicer基因的功能特征

Pengrong Huang^1,2,3,4,5, Huihui Yu^1,2,3,4,5, Muhammad Asad^1,2,3,4,5

¹State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.

Pest management science

|December 22, 2023

概括

在Plutella xylostella中,Dicer-1对发育和繁殖至关重要,而Dicer-2对RNA干扰至关重要. 这项研究澄清了这些Dicer基因在Lepidoptera中的作用.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
昆虫学昆虫学是一门学科.

背景情况:

迪克酶 (RNase III家族) 分裂双链RNA (dsRNA).
在昆虫中,Dicer-1 (Dcr-1) 和Dicer-2 (Dcr-2) 分别介导微RNA和小干扰RNA路径.
在Plutella xylostella中Dicer的特定功能以前是未知的.

研究的目的:

为了描述Plutella xylostella中的Dicer-1和Dicer-2基因.
阐明PxDcr-1和PxDcr-2在生物体中的功能作用.
了解Dicer基因在昆虫发育,繁殖和RNA干扰中的参与.

主要方法:

克隆和测序全长的Dicer-1和Dicer-2开放式阅读框架.
对Dicer蛋白的遗传学分析.
通过CRISPR-Cas9基因编辑,可以创建同卵性淘汰线 (ΔPxDcr-1和ΔPxDcr-2).
淘汰菌株的表型分析,包括生存,发育,繁殖和RNA干扰效率.

主要成果:

蛋白质PxDcr-1和PxDcr-2共享保留的结构域.
遗传学分析将PxDcr-1放在基层,而PxDcr-2则接近Lepidoptera中的Plodia interpunctella Dicer.
ΔPxDcr-1淘汰赛显示出高死亡率,化,产卵和化率降低,卵巢发育不足.
ΔPxDcr-2淘汰对生存,发育或繁殖没有显著影响,但大大降低了RNA干扰效率.

结论:

PxDcr-1对于Plutella xylostella的发育和繁殖至关重要,特别是在卵巢和卵形成中.
在这种物种中,PxDcr-2对于有效的RNA干扰是不可或缺的.
这些发现为Dicer-1和Dicer-2在Lepidoptera中的独特功能提供了关键的见解.

关键词:

克里斯普尔-Cas9是什么意思这就是说,Dicer.这种植物名为Plutella xylostella.这是一种RNA干扰.生殖和发展的繁殖和发展.

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