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牛乳腺微生物群的特征 微环境及其与体细胞计数的关联
Jing Liu1, Huan Liu1, Guangjie Cao1
1National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Veterinary sciences
|December 22, 2023
概括
乳牛的亚临床乳腺炎与牛奶微生物群多样性的变化有关. 较高的体细胞计数 (SCC) 与微生物多样性的减少和细菌群体的变化相关,这表明存在失生症.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物健康 动物健康
- 乳制品科学 乳制品科学
背景情况:
- 亚临床乳腺炎显著影响奶牛的健康和经济可行性.
- 体细胞计数 (SCC) 是评估乳腺炎严重程度的关键指标.
- 了解牛奶微生物组在乳腺炎中的作用对于群体管理至关重要.
研究的目的:
- 为了研究体细胞计数 (SCC),牛奶微生物群多样性和乳牛中亚临床乳腺炎之间的关系.
- 为了确定与不同SCC水平相关的特定微生物种群.
- 探索微生物失生症在亚临床乳腺炎的发展中的潜力.
主要方法:
- 16S rRNA基因的下一代测序用于不同SCC范围的牛奶样本.
- 微生物群落的组成和多样性与SCC值相关分析 (例如,2 × 10^5,5 × 10^5).
- 在SCC和特定细菌系的相对丰度之间进行了相关性分析.
主要成果:
- 当SCC超过2 × 10^5细胞/毫升时,牛奶微生物群的多样性显著下降.
- 细菌族群的组成随着SCC的增加而变化:Actinobacteriota在低SCC中占主导地位,而Firmicutes和Proteobacteria在高SCC中占主导地位.
- 罗姆布茨,图里西巴克特和帕尼克洛斯特里迪亚等特定属与SCC显示出正相关性,而斯塔菲洛科克,心理巴克特,阿洛科克和链球菌显示出负相关性.
结论:
- 亚临床乳腺炎的发展可能涉及乳腺失生症,而不仅仅是初级感染.
- 牛奶微生物组合的变化和减少的多样性与SCC的升高有关.
- 准微生物平衡可能为乳牛中亚临床乳腺炎的管理提供新的策略.
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