阴道:单层和集群形成-使用高分辨率扫描电子显微镜的超结构方面
Sharmila Fiama das Neves Ortiz1, Raphael Verdan1, Fabio da Silva de Azevedo Fortes2,3
1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Pesquisa em Medicina de Precisão, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil.
Pathogens (Basel, Switzerland)
|December 22, 2023
概括
阴道菌 (Trichomonas vaginalis) 形成了明显的类似上皮质的单层,具有强大的粘合力和具有脆弱附着的浮动集群. 这些结构不涉及细胞融合或寄生虫之间的分子转移.
科学领域:
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
- 微生物学 微生物学
背景情况:
- 阴道三虫 (Trichomonas vaginalis) 引起三病,这是一个广泛的性传播感染.
- 已知T. vaginalis对表面的附着性,但集群形成和膜相互作用的理解很少.
研究的目的:
- 为了研究T. vaginalis单层和集群在惰性基板上的形成.
- 为了比较单层与集群的超结构特征和粘附机制.
- 为了确定T. vaginalis单层是否表现出类似上皮的行为.
主要方法:
- 高分辨率扫描电子显微镜的扫描电子显微镜.
- 传输电子显微镜 (TEM) 和TEM断层扫描
- 细胞化学和染料注射技术
主要成果:
- 阴道T. vaginalis形成紧密的,类似上皮的单层,与培养瓶底部有强烈的交叉粘附.
- 漂浮的寄生虫聚合物 (集群) 表现出脆弱的附着物.
- 在两种形成类型中的附着细胞之间没有观察到细胞融合或分子交换.
结论:
- 在T. vaginalis中,单层和集群形成在粘附强度和结构上有显著差异.
- T. vaginalis单层表现出一些类似上皮的特征,但缺乏直接的细胞间通信途径,如融合或分子转移.
- 这些聚合过程不需要filopodia,cytonemes和细胞外囊泡.
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