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在瑞士发现的SARS-CoV-2核体蛋白突变扰乱了常用的多重RT-PCR试验中的N基因放大

Dominique Hilti^1,2, Faina Wehrli¹, Anna Roditscheff^2,3

¹Laboratory Dr. Risch, 9470 Buchs, Switzerland.

Pathogens (Basel, Switzerland)

|December 22, 2023

概括

在COVID-19 PCR测试中增加的N基因标失败与SARS-CoV-2 Delta变种有关. 全基因组测序发现了导致这些诊断失败的特定突变,突出显示了需要强大的变种检测方法的需要.

科学领域:

分子生物学分子生物学
病毒学病毒学
基因组学就是基因组学.

背景情况:

在使用TaqPath COVID-19 RT-PCR Kit时,观察到N基因目标失败 (NGTF) 的增加.
NGTF可以影响SARS-CoV-2检测和变种监测的准确性.

研究的目的:

为了调查使用TaqPath COVID-19 RT-PCR Kit观察到的增加NGTF的原因.
使用全基因组测序识别与NGTF相关的特定SARS-CoV-2突变.

主要方法:

使用牛津纳米孔技术进行全基因组测序.
对168,101个阳性SARS-CoV-2样本的分析,周期值 (CT) <30.
在N基因中核酸替代和删除的识别.

主要成果:

194个样本 (0.12%) 显示NGTF,主要是在三角洲变种浪潮期间.
在88.8%的NGTF样本中发现了核酸替代物G28922T (A217S).
在NGTF样本的子集中也发现了N基因的特定缺失.
完整的N基因放大与这些突变的缺失有关.

结论:

特定的SARS-CoV-2突变,特别是G28922T (A217S) 和N基因删除,在RT-PCR测定中负责NGTF.
由于病毒进化,N基因作为唯一的诊断目标的实用性可能会受到影响.
将RT-PCR与全基因组测序相结合对于识别诊断局限性和新变异至关重要.

关键词:

这就是SARS-CoV-2病毒.聚合酶连锁反应的发生.目标失败失败的目标失败进行全基因组测序.

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