野生类型的病原性生物膜去除潜力 乳酸菌cillus rhamnosus 菌株
Gregoria Mitropoulou1, Vasiliki Kompoura1, Grigorios Nelios1
1Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
Pathogens (Basel, Switzerland)
|December 22, 2023
概括
来自野生类型Lacticaseibacillus rhamnosus菌株的无细胞超体在去除致病性细菌生物膜方面显示出显著的潜力. 这些天然剂提供了对抗抗菌素耐药性和食品传播病原体的有希望的策略.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 公共卫生 公共卫生
背景情况:
- 抗菌素耐药性是全球主要的健康威胁.
- 细菌生物膜有助于病原体对抗微生物药物的耐药性.
- 乳酸细菌 (LAB) 和它们的代谢物在对抗生物膜方面表现有前途.
研究的目的:
- 为了评估从野生类型的Lacticaseibacillus rhamnosus菌株中获取的无细胞超水生物 (CFSs) 的生物膜去除能力.
- 为了比较希腊野生型菌株与商业L. rhamnosus GG菌株的疗效.
- 评估对常见的食源性病原体生物膜的活性:沙门氏菌,沙门氏菌Typhimurium,大肠杆菌,Listeria monocytogenes和黄金葡萄球菌.
主要方法:
- 在两天老的成熟生物膜上使用微板测试研究了生物膜的去除.
- 测试了来自五种野生型L. rhamnosus菌株和L. rhamnosus GG的非中和和中和CFS.
- 以度依赖的方式量化生物膜去除效率.
主要成果:
- 无论是中和还是非中和的CFS都表现出度依赖的生物膜去除.
- 与中和的CFS (30-80%) 相比,非中和的CFS表现出更高的疗效 (60-89%).
- 在野生型菌株中,L. rhamnosus OLXAL-3表现出最高的生物膜去除效率 (20-100%v/v).
结论:
- 野生类型的L. rhamnosus CFS具有重要的潜力,可以作为抗病原性细菌生物膜的天然剂.
- 这些发现突出了开发天然抗微生物药物的有希望的途径,以对抗食品传播病原体和抗微生物药物耐药性.
- 进一步研究这些CFS的应用可能会导致食品安全和公共卫生领域的新战略.
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