聚合物基纳米粒子与formoterol药物作为纳米载体系统在体外和实验性喘模型中的影响
Buket Bakan1,2, Anne-Charlotte Jonckheere3, Tatjana Decaesteker4
1Department of Public Health and Primary Care, Centre for Environment and Health, KU Leuven, 3000 Leuven, Belgium.
Toxics
|December 22, 2023
概括
用于喘治疗的聚合物纳米粒子在细胞测试中没有显示出毒性. 然而,在喘模型中,含有formoterol的纳米颗粒恶化了肺功能和炎症,需要进一步的安全测试.
科学领域:
- 纳米技术纳米技术
- 肺部药物输送 肺部药物输送
- 喘治疗方法 喘治疗方法
背景情况:
- 纳米技术为改善和向的肺部治疗提供了潜力.
- 纳米尺寸的药物载体必须是无毒的,并且没有不良影响,以确保安全的临床使用.
研究的目的:
- 在卵胺诱导喘模型中,评估在体外和体内素聚乙烯甲基酸盐纳米颗粒 (Lys-p(HEMA)) 载有形式醇的安全性和有效性.
主要方法:
- 使用16HBE和THP-1细胞系进行体外毒性评估.
- 在Balb/c小鼠模型中的卵胺诱导喘体内研究.
- 气道过敏反应 (AHR) 和肺炎 (细胞和生化) 在支气管支气管洗 (BAL) 中的评估.
主要成果:
- 这种Lys-p(HEMA) 纳米载体配方在体外表现出稳定的释放特征.
- 没有观察到体外毒性;细胞系在暴露于装载和卸载的纳米载体时保持活力.
- 在OVA喘模型中,自由形式醇和纳米颗粒配方都没有改善;与对照组相比,肺功能恶化和炎症增加.
结论:
- 装有formoterol的p(HEMA) 纳米载体在试验性喘模型中没有表现出体外毒性,但在试验性喘模型中加剧了肺功能障碍和炎症.
- 在临床应用这种含有formoterol的纳米载体用于肺部输送之前,需要进行广泛的进一步测试.
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