相关实验视频

Updated: Jul 7, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

奥克拉托克素A通过破坏铁的平衡和增加ROS诱导细胞铁亡

Sen Wang¹, Hui Ren¹, Chen Fan¹

¹International Cooperation Joint Laboratory of Jiangsu Province Colleges and Universities, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China.

Journal of agricultural and food chemistry

|December 22, 2023

概括

奥克拉托克素A (OTA) 通过破坏铁的平衡,导致细胞死亡,导致过度的氧化应激. 铁化有效地保护了细胞,揭示了铁.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
食品安全食品安全

背景情况:

毒素A (OTA) 是一种普遍存在的食品污染物,已知具有毒性作用.
导致OTA引起的损伤的确切机制,特别是氧化应激,仍然不完全理解.
细胞内铁失调与各种细胞死亡途径有关,包括铁亡.

研究的目的:

为了研究细胞内铁在甲素A诱导的活性氧物种 (ROS) 积累中的作用.
为了确定OTA是否会诱导人类细胞中依赖铁的ferroptotic细胞死亡.
为了阐明OTA对细胞铁平衡的影响.

主要方法:

已经使用的人类细胞系暴露于毒素A.
评估细胞活力,铁灭标志物和细胞内铁水平.
分析了铁代谢调节者的基因和蛋白质表达 (TFR1,FTH,FPN,Hepcidin).
在机械学研究中使用铁化剂和ROS吸尘器.

主要成果:

奥克拉托辛A治疗诱导了铁亡的特征和细胞活力降低.
通过对铁进口 (TFR1,FTH) 的上调和对铁出口 (FPN) 的下调,OTA扰乱了铁的稳态,增加了肝素.
在OTA暴露后观察到细胞内铁积累,ROS升高和脂质过氧化.
铁化显著改善了OTA诱导的毒性,铁过载和ROS产量.
ROS清理器部分逆转了OTA的影响,表明与铁失调相比,其作用是次要的.

结论:

奥克拉托克素A诱导细胞中的铁细胞死亡,主要是通过破坏铁的平衡.
由OTA诱导的细胞内铁积累是氧化应激和随后的细胞死亡的关键驱动因素.
准铁代谢是一种潜在的治疗策略,可以对抗甲毒素A的神经毒性.

关键词:

这就是OTA的意义.这就是ROSOS ROS.铁性化 (ferroptosis) 是一种铁是铁,铁是铁,铁是铁.氧化应激是一种氧化应激.

更多相关视频

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Published on: May 4, 2020

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

相关概念视频

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...

The Electron Transport Chain

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Anticholinesterase Agents: Poisoning and Treatment

Anticholinesterase Agents: Poisoning and Treatment

Anticholinesterases, also known as cholinesterase inhibitors, work by blocking the breakdown of acetylcholine, leading to its accumulation in the synaptic cleft. This accumulation indirectly enhances both muscarinic and nicotinic actions. These agents are classified as reversible or irreversible based on their mechanism of action.
Irreversible agents form a strong bond with the cholinesterase enzyme, making it inactive. The breakdown of the phosphorylated enzyme is...

Toxic Reactions: Overview

Toxic Reactions: Overview

When toxic substances penetrate the human body, they disseminate to various tissues, undergoing metabolic changes. This process yields reactive metabolites that may covalently bind with specific target molecules, resulting in toxicity.
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...

Electron Transport Chain: Complex III and IV

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...