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Updated: Jul 7, 2025

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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直接结合的方法来测量受体-质体相互作用

Manisha Ray1, Aryana Sayeed1, Madeline Ganshert1

  • 1Department of Chemistry and Biochemistry, Loyola University Chicago, 1068 W. Sheridan Road, Chicago, Illinois 60660, United States.

The journal of physical chemistry. B
|December 22, 2023
PubMed
概括

这项研究引入了一种新的无标签,自由溶液方法来测量G蛋白结合受体 (GPCR) 相互作用. 该技术量化了近本地条件的直接结合亲缘关系,推动了GPCR药物发现.

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Ligand Binding Sites

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Drug-receptor bonds are formed through various chemical forces when drugs interact with target cells. Covalent bonds, strong and irreversible, are exemplified by DNA-alkylating anticancer agents that inhibit cell division. However, such irreversible drug binding lacks selectivity and can modify the DNA of the surrounding healthy cells. Covalent binding often contributes to tissue toxicity, as seen with chloroform and paracetamol metabolites binding to the liver, causing hepatotoxicity.
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