侧面序列共写法调节了Twister的 ribozyme活动
Lauren N McKinley1,2, Reuben G Kern2,3, Sarah M Assmann2,4
1Depatment of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, United States.
Biochemistry
|December 22, 2023
概括
侧面序列可以通过形成不活跃的 ribozymogens 调节旋转的 ribozyme 活性. 这种调节可以通过外部控制,在基因表达和合成生物学方面具有潜力.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 在RNA生物学,RNA生物学.
背景情况:
- рибо酶是具有催化活性的RNA分子,但它们的调节机制比蛋白质酶的理解要少.
- 核分解性RNA的一种类型的Twister ribozymes具有已知的催化特性,但它们在原生序列中的调节还未得到充分研究.
- 自然存在的 ribozymes 不是孤立的,它们的活性可以受到相邻的基因组序列的影响.
研究的目的:
- 调查侧翼序列对米 (Oryza sativa) 中旋转核糖酶活性的调节作用.
- 为了识别特定的旋转核糖酶,其活性是由它们的原生基因组环境调节的.
- 探索适用于合成生物学的 ribozyme 调节和外部控制的潜在机制.
主要方法:
- 分析已发表的来自大米的RNA测序数据集.
- 结构探测技术用于研究RNA构造.
- 转录分离试验测量可测量 ribozyme 的活性.
- 以寡核酸为媒介的救援实验.
主要成果:
- 通过侧面序列观察到旋转核糖酶的差异调节.
- Osa 1-2 核酶与侧面序列没有相互作用.
- Osa 1-3 和 Osa 1-8 ribozymes 的侧面序列形成了不活跃的 ribozymogen 构造,减弱了自我裂变.
- 反感性寡核酸添加挽救了Osa 1-3 ribozymogen的活性.
结论:
- 侧翼序列在体内对旋转核糖酶活性进行差异调节方面发挥着重要作用.
- ribozymogen 形成代表了自然 ribozyme 调节的一个可信的机制.
- 通过反感性寡核酸体证明,对 ribozymogens 的外部控制对合成生物学和基因调节有影响.
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