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子使用对蛋白质结构和折叠的影响

McKenze J Moss¹, Laura M Chamness¹, Patricia L Clark¹

¹Department of Chemistry & Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA; email: mmoss6@nd.edu, lchamnes@nd.edu, pclark1@nd.edu.

Annual review of biophysics

|December 22, 2023

概括

同义代码子在合成过程中影响蛋白质折叠速率和机制. 了解蛋白质折叠中的子使用是至关重要的,但实验方法是有限的.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

蛋白质合成速率因同名编码子而异.
使用Codon可以调节协译蛋白折叠.
动态稳定的蛋白质具有复杂的折叠路径,对代码子的使用敏感.

研究的目的:

探索同名编码子替代在调节协译蛋白折叠中的潜力.
要突出在动力稳定蛋白质的折叠中使用子的重要性.
确定对实验方法的需求,以量化翻译延长和共同翻译折叠.

主要方法:

这项研究讨论了子使用对蛋白质折叠的理论含义.
它回顾了现有的关于蛋白质的文献,这些蛋白质具有对子使用敏感的折叠机制.
它强调缺乏用于细胞内定量化的实验技术.

主要成果:

在同源基因家族中保存的密码子使用模式表明选择用于折叠调节.
最初的蛋白质折叠回合的代介导调节可以产生长期影响.
对于动力稳定的蛋白质来说,编码子对蛋白质折叠的影响是显著的.

结论:

同义代码子在调节协译蛋白折叠方面发挥着重要作用.
需要进一步开发实验方法,以了解代介导的折叠调节.
目前,由于方法上的局限性,对编码子如何调节蛋白质折叠的预测性理解受到挑战.

关键词:

与翻译相关的蛋白质折叠.功能性蛋白质生产功能性蛋白质生产基因表达的基因表达方式非常罕见的鱼.同义词是代码子替代的代码子替代.翻译延长延长翻译

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