相关实验视频
Updated: Jul 7, 2025

14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
Published on: October 20, 2011
27.9K
在超高真空环境下进行表面成像和分析的低压扫描电子显微镜的形静电镜的设计和优化
Jeong-Woong Lee1, In-Yong Park2, Takashi Ogawa3
1Scientific Instruments Performance Evaluation Team, Advanced Instrumentation Institute, Korea Research Institute of Standards and Science (KRISS), 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Korea.
Ultramicroscopy
|December 22, 2023
概括
我们为超高真空低压扫描电子显微镜 (UHV LV-SEM) 设计了一种最佳的圆形静电镜头 (EOL). 这种镜头提高了空间分辨率和二次电子检测效率,用于表面分析.
科学领域:
- 材料科学 材料科学 材料科学
- 表面科学是一门学科.
- 电子显微镜电子显微镜
背景情况:
- 低压扫描电子显微镜 (LV-SEM) 可减少样本损伤,提高表面灵敏度.
- 超高真空 (UHV) 对于使用诸如奥格尔电子光谱 (AES) 和电子能量损失光谱 (EELS) 等技术进行表面元素分析至关重要.
- 现有的客观镜头可能很复杂,可能不适合UHV LV-SEM集成.
研究的目的:
- 为UHV LV-SEM应用设计和优化一个形静电目标镜头 (EOL).
- 为了实现高空间分辨率和高效的二次电子 (SE) 检测.
- 确保与超高频环境的兼容性,并促进光谱仪的集成.
主要方法:
- 使用模拟来优化三电极形EOL的设计和操作条件.
- 为了光谱仪集成和样本操纵,EOL的圆角被设置为60°.
- 基于偏差系数,探针直径和二次电子检测效率来评估性能.
主要成果:
- 优化的EOL在50 eV的着陆能量和1 mm的工作距离 (WD) 时实现了最小的球形 (0.05 mm) 和色谱 (0.03 mm) 偏差.
- 探头直径在1 keV时为2.3 nm,在50 eV时为5.7 nm (1 mm WD,10 pA探头电流).
- 在4毫米WD时,获得了83.3%的二次电子检测效率,证明了有效的信号采集.
结论:
- 优化的圆形EOL适用于UHV LV-SEM,提供高空间分辨率和高效的SE检测.
- 简单的,UHV兼容的设计可与表面分析的光谱仪进行集成.
- 这种镜头设计有可能推进用于表面成像和元素分析的高性能UHV LV-SEM系统.
相关概念视频
Transmission Electron Microscopy
5.5K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.5K
Preparation of Samples for Electron Microscopy
5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K
Overview of Electron Microscopy
9.2K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.2K
Scanning Electron Microscopy
4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
4.2K

