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一个敏感的"启动"的希夫基光探针用于选择性检测Fe3+和生物成像

Yiran Che¹, Jingying Yang¹, Zhenming Dong¹

¹School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|December 22, 2023

概括

开发了一种新的光探测器HENB,用于检测Fe3+离子. 这种探测器使细胞和斑马鱼中铁的敏感检测和成像成为可能,有助于铁亡研究.

科学领域:

化学传感器化学传感器
光探测器光探测器
生物医学应用生物医学应用

背景情况:

铁 (Fe3+) 在生物系统中至关重要,但其调节失调与ferroptosis等疾病有关.
开发用于Fe3+检测的选择性和敏感探针对于生物和医学研究至关重要.

研究的目的:

为了合成和描述一种新的Schiff基光探测器,HENB,用于Fe3+检测.
评估HENB在光谱传感,快速检测和Fe3+生物成像方面的表现.

主要方法:

希夫基光探针HENB的合成.
对HENB与Fe3+离子相互作用的光谱分析.
用于视觉检测Fe3+的测试条的制造.
光显微镜用于在活细胞和斑马鱼中成像Fe3+.

主要成果:

在与Fe3+离子结合时,HENB表现出选择性和敏感的光增强,低检测极限为4.51nM.
该探测器使用试管条便于用肉眼快速检测Fe3+ .
在活的Hela细胞和斑马鱼中,HENB成功地可视化了外源和内源Fe3+.
该探测器有效地监测了活细胞中铁灭过程中的Fe3+变化.

结论:

合成的HENB探头是用于敏感和选择性Fe3+检测和成像的有希望的工具.
HENB在活生物系统中监测Fe3+的能力,包括在ferroptosis期间,突出了其潜在的生物医学应用.

关键词:

检测检测检测检测检测铁 (Fe3+) 是一种铁.光探测器探测器可以发光.图像成像是一种成像.在这里,PET是PET.

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