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抑制定数感应是一种有效的策略,可以减轻土壤中人类细菌病原体的风险

Jinghan Zhang¹, Kun Lu¹, Lin Zhu¹

¹School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China.

Journal of hazardous materials

|December 22, 2023

概括

质量检测干扰剂有效减少有害细菌,毒性因子和抗生素耐药性基因. 这种环保策略还可以抑制基因转移,而不会损害有益的土壤微生物.

科学领域:

环境微生物学环境微生物学
细菌病原发生的细菌.
分子生物学分子生物学

背景情况:

携带抗生素耐药性基因 (ARG),移动遗传元素 (MGE) 和毒性因子基因 (VFG) 的人类细菌病原体 (HBPs) 构成重大生态和健康风险.
目前用于减轻这些风险的策略有限.
定数感应 (QS) 是一种关键的细菌通信系统,调节毒性和基因转移.

研究的目的:

调查QS干扰剂在减少HBPs和相关有害基因方面的有效性.
评估QS干扰对细菌通信,生物膜形成和水平基因转移的影响.
评估QS干扰剂的环境安全性.

主要方法:

用QS干扰剂对土壤样本的处理:4-尼托皮里丁-N-氧化物 (4-NPO) 和Acylase I.
量化HBP,VFG,ARG和MGE的数量.
评估细菌的沟通,生物膜的形成和结合频率.
对非致病性土壤微生物的影响分析.

主要成果:

干扰QS的药物显著降低了HBPs的48.30% (4-NPO) 和72.54% (乙酶I).
在HBPs的减少伴随着VFGs,ARGs和MGEs的水平降低.
质量系统干扰破坏了细菌的交流,抑制了生物膜的形成,并减少了水平基因转移频率.
没有观察到对其他土壤微生物代谢功能的显著不良影响.

结论:

质量系统干扰剂提供了一个有希望的,环保的策略来控制HBPs和减轻ARGs和VFGs的传播.
干扰细菌交流是一种有效的方法来减少病原体的丰富性和毒性.
这一策略尽量减少对生态安全和人类健康的风险,而不会破坏有益的微生物群落.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.横向基因转移是指水平基因转移.人类细菌病原体的病原体.议员数量检测是指对议员数量进行检测.检定人数感应干扰剂干扰剂

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