标签,你就是它:G-ICAT识别了p120-cysteine谷氨基化引发E-cadherin不稳定性的原因
Kevin T Fridianto1, Yimon Aye1
1Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland.
Cell chemical biology
|December 22, 2023
概括
一种名为G-ICAT的新方法量化了蛋白质谷氨基化. 它在p120-catenin上确定了特定的氨酸修饰,将其与细胞接触不稳定联系起来.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 目前的技术难以准确地测量单个蛋白质氨酸中的谷氨基化.
- 谷氨化是一种关键的翻译后修改,影响蛋白质功能.
- 了解特定的谷氨酸化位点对于破译细胞信号通路至关重要.
研究的目的:
- 引入一种新的方法,即基于谷氨的同位素编码亲和度标签 (G-ICAT),用于直接量化蛋白质谷氨化.
- 为了确定经过谷氨基化的特定的氨酸残留物及其功能后果.
- 为了研究谷氨酸化在细胞-细胞粘附的调节中的作用.
主要方法:
- 开发和应用G-ICAT技术.
- 基于质谱的蛋白质组学,以识别和量化甲.
- 分析p120-catenin及其与cadherin复合物的相互作用.
主要成果:
- 在特定的囊类残留物中,G-ICAT成功量化了谷氨基化.
- 在p120-catenin上的氨酸-692 (C692) 被确定为谷氨基化的一个位点.
- 在p120-catenin上C692的氨基化被证明可以促进E-cadherin的不稳定,影响细胞与细胞的接触.
结论:
- G-ICAT提供了一种强大的工具,用于量化特定位点的蛋白质谷氨.
- 氨酸-692对p120-catenin的氨酸化是维持细胞-细胞粘附的关键调节事件.
- 这一发现为基础细胞粘附调节的分子机制提供了新的见解.
相关概念视频
Catenins
2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Structure of Cadherins
3.3K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.3K
GPCRs Regulate Adenylyl Cylase Activity
5.6K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.6K


