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定量和绘制数字皮肤炎相关细菌在损伤和非损伤的身体部位和奶牛场环境的数量和地图

A P Dias¹, K Orsel¹, J De Buck¹

¹Faculty of Veterinary Medicine, University of Calgary, AB, T2N 4N1, Canada.

Journal of dairy science

|December 22, 2023

概括

数字性皮肤炎 (DD) 细菌储存库仍然不清楚. 这项研究发现了与DD相关的Treponema spp. 在病变中,健康的皮肤,唾液和受影响群体的环境,表明唾液和皮肤部位可能是关键来源.

科学领域:

兽医微生物学兽医微生物学
动物传染病动物传染病
牛的健康问题

背景情况:

数字性皮肤炎 (DD) 是乳牛的重要原因,影响动物福利和农场经济.
与DD相关的细菌的主要来源和传播途径尚未完全理解,这阻碍了有效的控制策略.

研究的目的:

在各种乳牛尸体地点和农场环境中识别数字皮肤炎 (DD) 细菌的潜在储存库.
调查与DD相关的特定细菌物种在受影响和不受影响的群体中的存在和分布.

主要方法:

一项涉及85头奶牛的横截面研究,来自5个群体 (3个有DD,2个没有).
采集皮肤拭子 (脚,,乳腺裂),唾液,尿液和奶牛便,以及环境样本.
实时定量PCR用于检测Treponema spp., Porphyromonas levii, Bacteroides pyogenes, Fusobacterium necrophorum和Fusobacterium mortiferum. 这三种细菌的真实时间定量PCR用于检测Treponema spp., Porphyromonas levii, Bacteroides pyogenes,Fusobacterium necrophorum和Fusobacterium mortiferum. 这三种细菌的真实时间定量PCR用于检测Treponema spp. 这三种细菌的真实时间定量PCR.

主要成果:

与DD有关的Treponema spp. 仅在受DD影响的群体,足部病变,健康的皮肤,唾液和环境样本中发现.
非Treponema spp. 其他类型. 在DD受影响和未受影响的群体中检测到.
在DD病变中,细菌负载高于健康皮肤,在裂病变中,细菌负载类似,这表明机会主义殖民.
唾液和/乌皮肤显示与DD-Treponema spp.有联系. 病变中的存在;便和尿液不是主要来源.

结论:

唾液和其他皮肤部位 (,裂) 可能在数字皮肤炎细菌的传播或持续中发挥作用.
便和尿液不太可能是DD相关细菌的主要储存库.
需要进行进一步的纵向研究,以了解这些潜在水库中DD相关细菌的动态,以告知控制措施.

关键词:

这是一个Treponemaema.脚病变脚病变脚病变传染病导致的.qPCRR 是一个很好的方法.

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