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在乳腺癌中,HER2蛋白水平与RNA范围量化的mRNA水平的相关性

Xiaoxian Li¹, Ji-Hoon Lee², Yuan Gao¹

¹Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

|December 22, 2023

概括

RNAscope准确地测量了乳腺癌中的HER2表达,优于目前对HER2低的病例的免疫组织化学 (IHC) 分析. 这种方法在识别可能对T-DXd治疗有反应的患者方面表现有前途.

科学领域:

在瘤学瘤学.
分子诊断学分子诊断
生物标志物研究生物标志物研究

背景情况:

特拉斯图祖马布德鲁克斯坦 (T-DXd) 已批准用于治疗HER2阳性和HER2低转移性乳腺癌.
目前的HER2免疫组织化学 (IHC) 测定难以准确评估HER2-低乳腺癌的T-DXd资格.
需要改进的方法来识别将受益于T-DXd治疗的患者.

研究的目的:

评估RNAscope作为一种用于量化乳腺癌中HER2表达的新型试验.
将RNAscope结果与现有的HER2 IHC测定 (PATHWAY和HercepTest) 进行比较.
通过RNAscope测量的HER2表达水平与患者对T-DXd治疗的反应相关联.

主要方法:

在526个乳腺癌组织微阵列核心中使用PATHWAY和HercepTest IHC试验评估HER2表达.
用RNAscope测量相同核中的HER2RNA水平.
在核心的子集中,使用免疫光学和质谱学量化HER2蛋白水平.
用RNAscope对23名接受T-DXd治疗的患者的转移性活检进行了测试,结果与应答率相关联.

主要成果:

根据RNAscope的HER2RNA水平与HER2蛋白水平和IHC H-分数 (P < .0001) 有很强的相关性.
对于IHC分数为0,超低和1+的蛋白质或RNA水平没有观察到显著差异.
在T-DXd响应者 (6.4点/细胞) 中发现了显著更高的RNA水平,相比于非响应者 (2.6点/细胞) (P = .030).

结论:

RNAscope是一种可靠且客观的测定方法,用于量化乳腺癌中HER2的表达.
RNAscope显示出作为当前IHC测试的替代方案的潜力,特别是在HER2低的乳腺癌中.
这种测试可能会改善患者选择特鲁苏马布德鲁克斯坦 (T-DXd) 治疗的情况.

关键词:

在HER2中,HER2是HER2.RNA范围的范围RNA范围的范围在T-DXd.生物标志物生物标志物预测性预测性预测性量化量化量化量化量化量化量化量化量化量化量化量化量

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