水力动力学力量对微生物燃料电池阳极中的电活性细菌粘附的影响
Alexiane Godain1,2, Timothy M Vogel2, Pascal Fongarland3
1Ecole Centrale de Lyon, INSA Lyon, Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Ampère, UMR5005, 69130 Ecully, France.
Bioengineering (Basel, Switzerland)
|December 23, 2023
概括
微生物燃料电池 (MFC) 中的剪切应力通过分离非EAB来选择性丰富电活性细菌 (EAB). 这一发现表明剪切应力可以增强阳极生物膜电活性.
科学领域:
- 微生物学 微生物学
- 生物电化学 生物电化学
- 环境工程 环境工程
背景情况:
- 细菌的附着是生物膜形成和电活性细菌 (EAB) 选择的关键.
- 水力动力学力,特别是剪切应力,显著影响细菌粘附.
- 剪切应力可能会选择性地分离非EAB,促进EAB的主导地位.
研究的目的:
- 研究在微生物燃料电池 (MFC) 中的阳极生物膜发育过程中剪切应力的选择EAB中的作用.
- 要确定剪切应力是否可以增强阳极上EAB的相对丰度.
主要方法:
- 构建的MFC反应堆具有剪切应力室,用于对阳极施加受控的剪切应力 (1, 10, 50 mPa).
- 使用16S rRNA基因测序比较微生物社区结构.
- 使用光显微镜进行量化生物膜覆盖.
主要成果:
- 高剪压 (50 mPa) 显著增加了EAB的相对丰度,如*Geobacter*,高达30%,与低剪压 (1%) 相比.
- 剪切应力降低了阳极上的整体生物膜覆盖率.
- 增加EAB的丰度与非EAB的脱落相关.
结论:
- 剪切应力作为选择性压力,在MFC阳极上的初始生物膜形成过程中有利于EAB.
- 这种机制增加了电活性物种的比例,可能会提高MFC的性能.
- 切削应力操纵为微生物电化学技术优化阳极生物膜提供了一种策略,尽管对成熟生物膜的影响需要进一步研究.
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