使用代谢工程Acetobacterium woodii菌株进行异质生产异醇
Franziska Höfele1, Teresa Schoch2, Catarina Oberlies2
1Institute of Molecular Biology and Biotechnology of Prokaryotes, Ulm University, 89081 Ulm, Germany.
Bioengineering (Basel, Switzerland)
|December 23, 2023
概括
乙性细菌被设计成从二氧化碳和中产生异醇. 这项研究优化了微生物发酵过程,使异醇产量增加了2.5倍.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 化石燃料的耗尽和二氧化碳的排放需要可持续的化学生产.
- 乙原体通过木材-Ljungdahl路径代谢CO或CO2 (+H2),提供了一个化石独立的替代方案.
- 异醇是一种广泛使用的工业化学品,推动了对可持续生产方法的需求.
研究的目的:
- 开发一种微生物发酵工艺,用于化石独立的异醇生产.
- 通过使用特定的酶,设计 *Acetobacterium woodii* 进行增强的异醇合成.
主要方法:
- 主要酶的基于等离子体的表达:酶A,CoA转移酶,乙乙酸脱碳酶和二次性酒精脱酶.
- 使用了来自 *Clostridium scatologenes*, *C. acetobutylicum* 和 *C. beijerinckii* 的基因.
- 优化了工程应变以克服路径瓶.
主要成果:
- 使用CO2 + H2.2实现了最大的5.64±1.08mM的异醇产量.
- 工程 * Acetobacterium woodii * 证明了成功的异醇合成.
- 压力优化导致异醇度增加了2.5倍.
结论:
- 从二氧化碳和H2中证明了微生物成功地产生异二醇.
- 工程制造的乙原体为可持续的异醇制造提供了一个可行的途径.
- 进一步优化有可能带来显著更高的产量.
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