在Pichia pastoris中调整脂肪酸概况和产量
Simon Kobalter1, Alena Voit1, Myria Bekerle-Bogner1
1Austrian Centre of Industrial Biotechnology (acib GmbH), Petersgasse 14, 8010 Graz, Austria.
Bioengineering (Basel, Switzerland)
|December 23, 2023
概括
代谢工程酵母Pichia pastoris现在可以从葡萄糖中产生超过1g/L的棕酸,一种有价值的脂肪酸. 这一进步为工业应用提供了可持续的非食品原料.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 传统的脂肪酸来源 (植物油,动物脂肪) 面临着可持续性挑战,因为全球对食品和工业原料的需求日益增加.
- 为了可持续的脂肪酸生产,正在探索农业副产品和二氧化碳等替代碳来源.
- 微生物发酵为生产特定脂肪酸提供了一个有前途的途径,但往往需要代谢工程才能有效地积累.
研究的目的:
- 通过代谢工程来设计Pichia pastoris酵母,使其从葡萄糖中产生高水平的棕酸和分泌棕酸.
- 识别和分析有益和有害的遗传修饰,以优化棕酸产量.
- 为了提高棕酸在产生的脂肪酸中的含量.
主要方法:
- 基因工程Pichia pastoris,包括基因删除 (脂肪乙-CoA合成酶) 和基因过度表达 (截断E. 大肠杆菌化酶" (TesA).
- 引入编码C16特异性∆9-脱和脂肪酸合成酶的基因,以量身定制脂肪酸配置文件.
- 优化种植条件,以改善脂肪酸的积累和分泌.
主要成果:
- 经过工程改造的Pichia pastoris菌株在培养基中分泌了超过1g/L的自由脂肪酸.
- 代谢工程策略导致棕酸含量显著增加,从5.5%增加到22%.
- 确定增强脂肪酸分泌和特定脂肪酸成分的关键遗传修饰.
结论:
- 牧羊是微生物生产棕酸的可行的宿主,使用像葡萄糖这样的可持续原料.
- 代谢工程方法,包括异质基因的表达和途径优化,对于生产特定脂肪酸是有效的.
- 这项工作证明了向可持续的非食品工业脂肪酸生产迈出的重要一步.
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