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生理学和转录组分析外源性菌素对胡幼苗干旱反应的影响

Huangying Shu^1,2,3, Muhammad Ahsan Altaf^1,2, Naveed Mushtaq^1,2

¹Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Sanya 572025, China.

Antioxidants (Basel, Switzerland)

|December 23, 2023

概括

斯特里戈拉克顿 (SL) 应用通过改善水含量,根生长和色素水平来提高胡植物对干旱压力的弹性. 它还调节基因表达,为抗旱的胡种植提供了策略.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
农业科学农业科学

背景情况:

干旱压力严重影响胡作物的产量和质量.
斯特里戈拉克 (SL) 显示出减轻干旱影响的潜力,但其在胡中的分子机制尚未完全理解.

研究的目的:

研究通过叶子应用SL增强胡幼苗干旱耐受性的生理和分子机制.
确定关键的基因和途径,参与SL介导的干旱应激反应.

主要方法:

生理学评估包括生长,相对含水量 (RWC),抗氧化酶活性和氧化损伤标志物.
转录组分析 (RNA-Seq) 来识别差异表达的基因.
定量实时PCR (qRT-PCR) 用于验证基因表达.

主要成果:

SL的应用改善了RWC,根形态和叶色素,同时减少了氧化损伤 (MDA,H2O2) 并保持了抗氧化酶活性 (POD,CAT,SOD).
转录组分析揭示了核糖体中的基因丰富,ABC载体,烯胺生物合成和Auxin/MAPK信号通路.
在干旱期间,在SL治疗下,AGR7,ABI5,BRI1和PDR4等关键基因被上调,而SAPK6,NTF4,PYL6,GPX4和SMXL6则被下调.

结论:

通过生理上的改善和特定基因表达路径的调制,SL有效地减轻了胡植物的干旱压力.
了解这些分子机制为开发耐旱的胡品种提供了战略.

关键词:

干旱造成的压力是干旱.一个分子的分子分子分子.胡胡胡胡是一个这种药物被称为strigolactone.转录组 (transcriptome) 是一个转录组.

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