乳酸通过GPR81-ERK1/2通路调解高强度间歇训练诱导的海马线粒体功能的促进
Qinghui Shang1,2, Xuepeng Bian2, Lutao Zhu2
1Key Laboratory of Exercise and Health Sciences, Shanghai University of Sport, Ministry of Education, Shanghai 200438, China.
Antioxidants (Basel, Switzerland)
|December 23, 2023
概括
高强度间歇训练 (HIIT) 通过乳酸和GPR81-ERK1/2通路促进大脑线粒体功能. 这增强了突触可塑性和ATP生产,这对认知健康至关重要.
科学领域:
- 神经科学是一个神经科学.
- 线粒体生物学 线粒体生物学
- 运动生理学 运动生理学
背景情况:
- 线粒体生物发生和融合对于细胞的能量和功能至关重要.
- 高强度间歇训练 (HIIT) 改善海马线粒体功能,但机制尚不清楚.
- 在HIIT期间产生的乳酸可能会激活GPR81受体,影响神经可塑性和线粒体健康.
研究的目的:
- 阐明HIIT增强海马线粒体功能的机制.
- 研究乳酸和GPR81受体在调解HIIT效应中的作用.
- 为了确定ERK1/2信号通路的参与.
主要方法:
- 在体内:使用AAV载体在小鼠海马中敲除GPR81,然后进行HIIT.
- 在体外:Neuro-2A细胞用L-乳酸盐,GPR81激动剂和ERK1/2抑制剂进行治疗.
- 评估了线粒体生物发生,融合,ATP生产,氧化酸化 (OXPHOS) 和突触可塑性.
主要成果:
- 在野生型小鼠中,HIIT增加了线粒体数量,ATP生产,OXPHOS,线粒体生物发生,融合和突触可塑性.
- 这些HIIT诱导的好处在GPR81敲击小鼠中被废除了.
- 在体外,L-乳酸盐和GPR81激动剂治疗促进了线粒体功能和突触可塑性,这些影响取决于ERK1/2激活.
结论:
- 乳酸调解了HIIT对海马线粒体功能的积极影响.
- GPR81-ERK1/2信号通路对于HIIT诱导的线粒体适应和突触可塑性至关重要.
- 向乳酸-GPR81-ERK1/2轴可能为认知增强提供治疗策略.
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