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相关概念视频

CRISPR01:59

CRISPR

51.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jul 7, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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gRNA设计:它的进化如何影响CRISPR/Cas9系统的改进.

Cristofer Motoche-Monar1, Julián E Ordoñez1, Oscar Chang2,3

  • 1School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí 100119, Ecuador.

Biomolecules
|December 23, 2023
PubMed
概括

本研究回顾了CRISPR/Cas9指导RNA (gRNA) 设计工具,追踪了它们在计算架构和特征中的演变. 它强调了机器学习和神经网络,以优化基因工程中的gRNA设计.

关键词:
这就是CRISPR/Cas9的作用.深度学习是一种深度学习.在gRNARNA中.机器学习是机器学习.神经网络的神经网络的神经网络

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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A Customizable Protocol for String Assembly gRNA Cloning STAgR
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A Customizable Protocol for String Assembly gRNA Cloning STAgR

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Last Updated: Jul 7, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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A Customizable Protocol for String Assembly gRNA Cloning STAgR

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 细菌防御机制CRISPR/Cas9系统已经被彻底改变为精确的基因组编辑工具.
  • 克里斯普尔系统提供了对细菌的免疫力,但可以在各种生物体中进行精确的基因组编辑.

研究的目的:

  • 为CRISPR/Cas9.9提供历史和最近基于Web的指导RNA (gRNA) 设计工具的概述.
  • 突出这些工具中计算架构和gRNA特征的演变.
  • 强调gRNA设计在CRISPR/Cas9系统的准确性能方面的重要性.

主要方法:

  • 对gRNA设计的现有文献和基于Web的工具进行审查.
  • 分析计算方法,包括机器学习和神经网络.
  • 检查gRNA/目标相互作用数据用于预测和分类.

主要成果:

  • gRNA设计工具的演变显示了计算架构和gRNA特征的进步.
  • 机器学习和神经网络越来越多地用于预测和分类有效的gRNA.
  • 目前的工具利用gRNA/目标交互数据来提高设计准确性.

结论:

  • 优化的gRNA设计对于CRISPR/Cas9技术的成功应用至关重要.
  • 计算方法和算法的进步对于开发更好的gRNAs至关重要.
  • 本次审查旨在培养一个专注于利用最新的算法用于现代CRISPR/Cas9工程的社区.